4rub

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[[Image:4rub.gif|left|200px]]
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{{STRUCTURE_4rub| PDB=4rub | SCENE= }}
{{STRUCTURE_4rub| PDB=4rub | SCENE= }}
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'''A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE'''
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===A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE===
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==Overview==
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A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) from Nicotiana tabacum has been obtained at alkaline pH with polyethylene glycol 8000 in the presence of a non-ionic detergent, beta-octyl glucoside. The crystals are grown at room temperature by the hanging-drop vapor diffusion technique from a protein solution containing enzyme complexed with CO2, Mg2+, and the transition state analog 2-C-carboxy-D-arabinitol-1,5-bisphosphate. The crystals belong to the the space group P3(1)21 (or P3(2)21) with the cell parameters a = 204.6 A, and c = 117.4 A (1 A = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 A Bragg spacing and are suitable for X-ray structure determination.
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(as it appears on PubMed at http://www.pubmed.gov), where 3681999 is the PubMed ID number.
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{{ABSTRACT_PUBMED_3681999}}
==About this Structure==
==About this Structure==
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[[Category: Eisenberg, D.]]
[[Category: Eisenberg, D.]]
[[Category: Schreuder, H.]]
[[Category: Schreuder, H.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:29:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:49:52 2008''

Revision as of 10:49, 3 July 2008

Image:4rub.png

Template:STRUCTURE 4rub

A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE

Template:ABSTRACT PUBMED 3681999

About this Structure

4RUB is a Protein complex structure of sequences from Nicotiana tabacum. Full crystallographic information is available from OCA.

Reference

A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state., Suh SW, Cascio D, Chapman MS, Eisenberg D, J Mol Biol. 1987 Sep 20;197(2):363-5. PMID:3681999

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