5gcn

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[[Image:5gcn.gif|left|200px]]
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{{STRUCTURE_5gcn| PDB=5gcn | SCENE= }}
{{STRUCTURE_5gcn| PDB=5gcn | SCENE= }}
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'''CATALYTIC DOMAIN OF TETRAHYMENA GCN5 HISTONE ACETYLTRANSFERASE IN COMPLEX WITH COENZYME A'''
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===CATALYTIC DOMAIN OF TETRAHYMENA GCN5 HISTONE ACETYLTRANSFERASE IN COMPLEX WITH COENZYME A===
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==Overview==
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Gene transcription requires the release of inactive DNA from its packaging of histone proteins. Following the discovery of the first transcription-associated histone acetyltransferase, tetrahymena GCN5, it was shown that yeast GCN5 is recruited to the promoter and causes hyper-acetylation of histones and transcriptional activation of target genes, establishing a direct connection between histone acetylation and transcriptional activation. Many other important transcription regulators have been found to have histone acetyltransferase activity, including TAFII230/250, p300/CBP and its associated factor PCAF. Here we present the solution structure of the catalytic domain of tGCN5 (residues 47-210) in complex with coenzyme A. The structure contains two domains; the amino-terminal domain is similar to those of other GCN5-related N-acetyltransferases but the carboxy-terminal domain is not. Coenzyme A binds in a deep hydrophobic pocket between the two domains. Chemical shift changes upon titration with histone H3 peptides indicate a binding site at the domain boundary opposite to the coenzyme A site. The structural data indicate a single-step acetyl-transfer reaction mechanism catalysed by a hydrogen bond to the backbone amide group of leucine 126 and the side-chain carboxyl group of a conserved acidic residue.
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{{ABSTRACT_PUBMED_10403255}}
==About this Structure==
==About this Structure==
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5GCN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GCN OCA].
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5GCN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GCN OCA].
==Reference==
==Reference==
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[[Category: Histone acetyltransferase]]
[[Category: Histone acetyltransferase]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:34:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 14:03:00 2008''

Revision as of 11:03, 3 July 2008

Image:5gcn.png

Template:STRUCTURE 5gcn

CATALYTIC DOMAIN OF TETRAHYMENA GCN5 HISTONE ACETYLTRANSFERASE IN COMPLEX WITH COENZYME A

Template:ABSTRACT PUBMED 10403255

About this Structure

5GCN is a Single protein structure of sequence from Tetrahymena thermophila. Full experimental information is available from OCA.

Reference

Solution structure of the catalytic domain of GCN5 histone acetyltransferase bound to coenzyme A., Lin Y, Fletcher CM, Zhou J, Allis CD, Wagner G, Nature. 1999 Jul 1;400(6739):86-9. PMID:10403255

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