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7cgt

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{{STRUCTURE_7cgt| PDB=7cgt | SCENE= }}
{{STRUCTURE_7cgt| PDB=7cgt | SCENE= }}
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'''RAMEB COMPLEX OF CYCLODEXTRIN GLYCOSYLTRANSFERASE MUTANT'''
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===RAMEB COMPLEX OF CYCLODEXTRIN GLYCOSYLTRANSFERASE MUTANT===
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==Overview==
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Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic maltooligosaccharides (cyclodextrins) which are of interest in various applications. The cyclization reaction gives rise to a spectrum of ring sizes consisting of predominantly six to eight glucosyl units. Using the enzyme from Bacillus circulans strain no. 8, binding studies have been performed with several substrates and analogues. The observed binding modes differ in detail, but agree in general with data on homologous enzymes. Based on these binding studies, two mutations were designed that changed the production spectrum from the predominant product beta-cyclodextrin of the wild-type enzyme towards gamma-cyclodextrin, which is of practical interest because it is rare and can encapsulate larger nonpolar compounds.
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(as it appears on PubMed at http://www.pubmed.gov), where 9738912 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9738912}}
==About this Structure==
==About this Structure==
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[[Category: Glycosyltransferase]]
[[Category: Glycosyltransferase]]
[[Category: Starch degradation]]
[[Category: Starch degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:44:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 14:31:34 2008''

Revision as of 11:31, 3 July 2008

Image:7cgt.png

Template:STRUCTURE 7cgt

RAMEB COMPLEX OF CYCLODEXTRIN GLYCOSYLTRANSFERASE MUTANT

Template:ABSTRACT PUBMED 9738912

About this Structure

7CGT is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.

Reference

Substrate binding to a cyclodextrin glycosyltransferase and mutations increasing the gamma-cyclodextrin production., Parsiegla G, Schmidt AK, Schulz GE, Eur J Biochem. 1998 Aug 1;255(3):710-7. PMID:9738912

Page seeded by OCA on Thu Jul 3 14:31:34 2008

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