2rfc

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{{STRUCTURE_2rfc| PDB=2rfc | SCENE= }}
{{STRUCTURE_2rfc| PDB=2rfc | SCENE= }}
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'''Ligand bound (4-phenylimidazole) Crystal Structure of a Cytochrome P450 from the Thermoacidophilic Archaeon Picrophilus Torridus'''
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===Ligand bound (4-phenylimidazole) Crystal Structure of a Cytochrome P450 from the Thermoacidophilic Archaeon Picrophilus Torridus===
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==Overview==
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The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phenylimidazole. Comparison of the ligand-free and -bound CYP231A2 structures shows conformational changes where the F and G helices swing as a single rigid body about a pivot point at the N-terminal end of the F helix, allowing the F helix region to dip toward the heme, resulting in closer contacts with the ligand. Thermal melting data illustrate that the melting temperature for CYP231A2 increases nearly 10 degrees C upon ligand binding, thus illustrating that the closed conformation is substantially more stable. Furthermore, spectroscopic data indicate that the active site is stable at pH 4.5, although, unusually, the thiolate ligand to the iron can be reversibly protonated. CYP231A2 does not exhibit structural features normally associated with thermophilic proteins such as an increase in salt bridge networks or extensive aromatic clustering. The increase in thermal stability instead is best correlated with the smaller size and shorter loops in CYP231A2 compared to other P450s.
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(as it appears on PubMed at http://www.pubmed.gov), where 18197710 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18197710}}
==About this Structure==
==About this Structure==
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[[Category: Monooxygenase]]
[[Category: Monooxygenase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:48:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat Jul 5 16:25:40 2008''

Revision as of 13:25, 5 July 2008

Image:2rfc.png

Template:STRUCTURE 2rfc

Ligand bound (4-phenylimidazole) Crystal Structure of a Cytochrome P450 from the Thermoacidophilic Archaeon Picrophilus Torridus

Template:ABSTRACT PUBMED 18197710

About this Structure

2RFC is a Single protein structure of sequence from Picrophilus torridus. Full crystallographic information is available from OCA.

Reference

Crystal Structure and Properties of CYP231A2 from the Thermoacidophilic Archaeon Picrophilus torridus(,)., Ho WW, Li H, Nishida CR, Montellano PR, Poulos TL, Biochemistry. 2008 Feb 19;47(7):2071-9. Epub 2008 Jan 16. PMID:18197710

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