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9dna

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{{STRUCTURE_9dna| PDB=9dna | SCENE= }}
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'''CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)'''
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===CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)===
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==Overview==
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Single crystals of the self-complementary octadeoxyribonucleotide d(GCCCGGGC) have been analysed by X-ray diffraction methods at a resolution of 1.8 A. The tetragonal unit cell of space group P4(3)2(1)2 has dimensions of a = 43.25 A and c = 24.61 A and contains eight strands of the oligonucleotide. The structure was refined by standard crystallographic techniques to an R factor of 17.1% using 1359 3 sigma structure factor observations. Two strands of the oligonucleotide are related by the crystallographic dyad axis to form a DNA helix in the A conformation. The d(GCCCGGGC) helix is characterized by a wide open major groove, a near perpendicular orientation of base pairs to the helix axis and an unusually small average helix twist angle of 31.3 degrees indicating a slightly underwound helix with 11.5 base pairs per turn. Extensive cross-strand stacking between guanine bases at the central cytosine-guanine step is made possible by a number of local conformational adjustments including a fully extended sugar-phosphate backbone of the central guanosine nucleotide.
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(as it appears on PubMed at http://www.pubmed.gov), where 3684603 is the PubMed ID number.
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{{ABSTRACT_PUBMED_3684603}}
==About this Structure==
==About this Structure==
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[[Category: A-dna]]
[[Category: A-dna]]
[[Category: Double helix]]
[[Category: Double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:52:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 6 00:59:48 2008''

Revision as of 21:59, 5 July 2008

Image:9dna.png

Template:STRUCTURE 9dna

CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)

Template:ABSTRACT PUBMED 3684603

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure analysis of an A-DNA fragment at 1.8 A resolution: d(GCCCGGGC)., Heinemann U, Lauble H, Frank R, Blocker H, Nucleic Acids Res. 1987 Nov 25;15(22):9531-50. PMID:3684603

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