2nlw

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{{STRUCTURE_2nlw| PDB=2nlw | SCENE= }}
{{STRUCTURE_2nlw| PDB=2nlw | SCENE= }}
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'''Solution structure of the RRM domain of human eukaryotic initiation factor 3b'''
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===Solution structure of the RRM domain of human eukaryotic initiation factor 3b===
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==Overview==
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Mammalian eIF3 is a 700-kDa multiprotein complex essential for initiation of protein synthesis in eukaryotic cells. It consists of 13 subunits (eIF3a to -m), among which eIF3b serves as a major scaffolding protein. Here we report the solution structure of the N-terminal RNA recognition motif of human eIF3b (eIF3b-RRM) determined by NMR spectroscopy. The structure reveals a noncanonical RRM with a negatively charged surface in the beta-sheet area contradictory with potential RNA binding activity. Instead, eIF3j, which is required for stable 40 S ribosome binding of the eIF3 complex, specifically binds to the rear alpha-helices of the eIF3b-RRM, opposite to its beta-sheet surface. Moreover, we identify that an N-terminal 69-amino acid peptide of eIF3j is sufficient for binding to eIF3b-RRM and that this interaction is essential for eIF3b-RRM recruitment to the 40 S ribosomal subunit. Our results provide the first structure of an important subdomain of a core eIF3 subunit and detailed insights into protein-protein interactions between two eIF3 subunits required for stable eIF3 recruitment to the 40 S subunit.
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(as it appears on PubMed at http://www.pubmed.gov), where 17190833 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17190833}}
==About this Structure==
==About this Structure==
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2NLW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NLW OCA].
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2NLW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NLW OCA].
==Reference==
==Reference==
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[[Category: Rna recognition motif]]
[[Category: Rna recognition motif]]
[[Category: Translation initiation]]
[[Category: Translation initiation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:37:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 9 10:06:11 2008''

Revision as of 07:06, 9 July 2008

Image:2nlw.png

Template:STRUCTURE 2nlw

Solution structure of the RRM domain of human eukaryotic initiation factor 3b

Template:ABSTRACT PUBMED 17190833

About this Structure

2NLW is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit., ElAntak L, Tzakos AG, Locker N, Lukavsky PJ, J Biol Chem. 2007 Mar 16;282(11):8165-74. Epub 2006 Dec 26. PMID:17190833

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