This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2nlw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2nlw.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2nlw.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2nlw| PDB=2nlw | SCENE= }}
{{STRUCTURE_2nlw| PDB=2nlw | SCENE= }}
-
'''Solution structure of the RRM domain of human eukaryotic initiation factor 3b'''
+
===Solution structure of the RRM domain of human eukaryotic initiation factor 3b===
-
==Overview==
+
<!--
-
Mammalian eIF3 is a 700-kDa multiprotein complex essential for initiation of protein synthesis in eukaryotic cells. It consists of 13 subunits (eIF3a to -m), among which eIF3b serves as a major scaffolding protein. Here we report the solution structure of the N-terminal RNA recognition motif of human eIF3b (eIF3b-RRM) determined by NMR spectroscopy. The structure reveals a noncanonical RRM with a negatively charged surface in the beta-sheet area contradictory with potential RNA binding activity. Instead, eIF3j, which is required for stable 40 S ribosome binding of the eIF3 complex, specifically binds to the rear alpha-helices of the eIF3b-RRM, opposite to its beta-sheet surface. Moreover, we identify that an N-terminal 69-amino acid peptide of eIF3j is sufficient for binding to eIF3b-RRM and that this interaction is essential for eIF3b-RRM recruitment to the 40 S ribosomal subunit. Our results provide the first structure of an important subdomain of a core eIF3 subunit and detailed insights into protein-protein interactions between two eIF3 subunits required for stable eIF3 recruitment to the 40 S subunit.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17190833}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17190833 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17190833}}
==About this Structure==
==About this Structure==
-
2NLW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NLW OCA].
+
2NLW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NLW OCA].
==Reference==
==Reference==
Line 29: Line 33:
[[Category: Rna recognition motif]]
[[Category: Rna recognition motif]]
[[Category: Translation initiation]]
[[Category: Translation initiation]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:37:27 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 9 10:06:11 2008''

Revision as of 07:06, 9 July 2008

Image:2nlw.png

Template:STRUCTURE 2nlw

Solution structure of the RRM domain of human eukaryotic initiation factor 3b

Template:ABSTRACT PUBMED 17190833

About this Structure

2NLW is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit., ElAntak L, Tzakos AG, Locker N, Lukavsky PJ, J Biol Chem. 2007 Mar 16;282(11):8165-74. Epub 2006 Dec 26. PMID:17190833

Page seeded by OCA on Wed Jul 9 10:06:11 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2nlw"
Personal tools