1bvw
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(New page: 200px<br /> <applet load="1bvw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bvw, resolution 1.92Å" /> '''CELLOBIOHYDROLASE I...)
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Revision as of 18:00, 29 October 2007
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CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS
Overview
The three-dimensional structure of the catalytic core of the family 6, cellobiohydrolase II, Cel6A (CBH II), from Humicola insolens has been, determined by X-ray crystallography at a resolution of 1.92 A. The, structure was solved by molecular replacement using the homologous, Trichoderma reesei CBH II as a search model. The H. insolens enzyme, displays a high degree of structural similarity with its T. reesei, equivalent. The structure features both O- (alpha-linked mannose) and, N-linked glycosylation and a hexa-co-ordinate Mg2+ ion. The active-site, residues are located within the enclosed tunnel that is typical for, cellobiohydrolase enzymes and which may permit a processive hydrolysis of, the cellulose substrate. The close structural similarity between the two, enzymes implies that ... [(full description)]
About this Structure
1BVW is a [Single protein] structure of sequence from [Humicola insolens] with MAN, NAG, MG and GOL as [ligands]. Active as [[1]], with EC number [3.2.1.91]. Full crystallographic information is available from [OCA].
Reference
Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 A resolution., Varrot A, Hastrup S, Schulein M, Davies GJ, Biochem J. 1999 Jan 15;337 ( Pt 2):297-304. PMID:9882628
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