2gpm

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{{STRUCTURE_2gpm| PDB=2gpm | SCENE= }}
{{STRUCTURE_2gpm| PDB=2gpm | SCENE= }}
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'''Crystal structure of an RNA racemate'''
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===Crystal structure of an RNA racemate===
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==Overview==
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The racemate of the RNA duplex r(CUGGGCGG).r(CCGCCUGG) from Thermus flavus 5S rRNA has been crystallized and examined by X-ray crystallography. The space group is P1(_) with approximate unit-cell parameters a = 26.5, b = 38.0, c = 45.4 Angstrom, alpha = 113.1, beta = 100.5, gamma = 93.3 degrees. The structure was solved by molecular replacement. There are four RNA duplexes in the unit cell. The crystal lattice consists of columns of RNA duplexes. The duplexes are stacked end-to-end and are stabilized by intermolecular base-stacking interactions. Within each column the L-duplexes and D-duplexes are stacked alternately. Every other duplex in each stack has two alternative conformations, approximately equally occupied, corresponding to molecules oriented in opposite directions. Neighbouring columns are related by the crystallographic centre of symmetry. The unit cell also contains approximately 250 ordered water molecules and six ordered calcium ions. A glycerol molecule is visible in the minor groove interacting with a guanosine residue.
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The line below this paragraph, {{ABSTRACT_PUBMED_16699193}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16699193 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16699193}}
==About this Structure==
==About this Structure==
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[[Category: Double helix]]
[[Category: Double helix]]
[[Category: Racemate]]
[[Category: Racemate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:22:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 10 12:32:39 2008''

Revision as of 09:32, 10 July 2008

Image:2gpm.png

Template:STRUCTURE 2gpm

Crystal structure of an RNA racemate

Template:ABSTRACT PUBMED 16699193

About this Structure

Full crystallographic information is available from OCA.

Reference

The first crystal structure of an RNA racemate., Rypniewski W, Vallazza M, Perbandt M, Klussmann S, Delucas LJ, Betzel C, Erdmann VA, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):659-64. Epub 2006, May 12. PMID:16699193

Page seeded by OCA on Thu Jul 10 12:32:39 2008

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