1j4j

From Proteopedia

---

Revision as of 15:48, 20 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1j4j, resolution 2.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of Tabtoxin Resistance Protein (form II) complexed with an Acyl Coenzyme A

Overview

Tabtoxin resistance protein (TTR) is an enzyme that renders, tabtoxin-producing pathogens, such as Pseudomonas syringae, tolerant to, their own phytotoxins. Here, we report the crystal structure of TTR, complexed with its natural cofactor, acetyl coenzyme A (AcCoA), to 1.55A, resolution. The binary complex forms a characteristic "V" shape for, substrate binding and contains the four motifs conserved in the, GCN5-related N-acetyltransferase (GNAT) superfamily, which also includes, the histone acetyltransferases (HATs). A single-step mechanism is proposed, to explain the function of three conserved residues, Glu92, Asp130 and, Tyr141, in catalyzing the acetyl group transfer to its substrate. We also, report that TTR possesses HAT activity and suggest an evolutionary, relationship between TTR and other GNAT members.

About this Structure

1J4J is a Single protein structure of sequence from Pseudomonas syringae pv. tabaci with ACO as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of tabtoxin resistance protein complexed with acetyl coenzyme A reveals the mechanism for beta-lactam acetylation., He H, Ding Y, Bartlam M, Sun F, Le Y, Qin X, Tang H, Zhang R, Joachimiak A, Liu J, Zhao N, Rao Z, J Mol Biol. 2003 Jan 31;325(5):1019-30. PMID:12527305

Page seeded by OCA on Tue Nov 20 17:55:48 2007