1zbl

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{{Seed}}
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{{STRUCTURE_1zbl| PDB=1zbl | SCENE= }}
{{STRUCTURE_1zbl| PDB=1zbl | SCENE= }}
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'''Bacillus halodurans RNase H catalytic domain mutant D192N in complex with 12-mer RNA/DNA hybrid'''
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===Bacillus halodurans RNase H catalytic domain mutant D192N in complex with 12-mer RNA/DNA hybrid===
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==Overview==
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RNase H belongs to a nucleotidyl-transferase superfamily, which includes transposase, retroviral integrase, Holliday junction resolvase, and RISC nuclease Argonaute. We report the crystal structures of RNase H complexed with an RNA/DNA hybrid and a mechanism for substrate recognition and two-metal-ion-dependent catalysis. RNase H specifically recognizes the A form RNA strand and the B form DNA strand. Structure comparisons lead us to predict the catalytic residues of Argonaute and conclude that two-metal-ion catalysis is a general feature of the superfamily. In nucleases, the two metal ions are asymmetrically coordinated and have distinct roles in activating the nucleophile and stabilizing the transition state. In transposases, they are symmetrically coordinated and exchange roles to alternately activate a water and a 3'-OH for successive strand cleavage and transfer by a ping-pong mechanism.
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(as it appears on PubMed at http://www.pubmed.gov), where 15989951 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15989951}}
==About this Structure==
==About this Structure==
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[[Category: Rna/dna hybrid]]
[[Category: Rna/dna hybrid]]
[[Category: Rnase h]]
[[Category: Rnase h]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:25:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 10 12:35:02 2008''

Revision as of 09:35, 10 July 2008

Image:1zbl.png

Template:STRUCTURE 1zbl

Bacillus halodurans RNase H catalytic domain mutant D192N in complex with 12-mer RNA/DNA hybrid

Template:ABSTRACT PUBMED 15989951

About this Structure

1ZBL is a Single protein structure of sequence from Bacillus halodurans. Full crystallographic information is available from OCA.

Reference

Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis., Nowotny M, Gaidamakov SA, Crouch RJ, Yang W, Cell. 2005 Jul 1;121(7):1005-16. PMID:15989951

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