1q9y

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{{STRUCTURE_1q9y| PDB=1q9y | SCENE= }}
{{STRUCTURE_1q9y| PDB=1q9y | SCENE= }}
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'''CRYSTAL STRUCTURE OF ENTEROBACTERIA PHAGE RB69 GP43 DNA POLYMERASE COMPLEXED WITH 8-OXOGUANOSINE CONTAINING DNA'''
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===CRYSTAL STRUCTURE OF ENTEROBACTERIA PHAGE RB69 GP43 DNA POLYMERASE COMPLEXED WITH 8-OXOGUANOSINE CONTAINING DNA===
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==Overview==
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The initial encounter of an unrepaired DNA lesion is likely to be with a replicative DNA polymerase, and the outcome of this event determines whether an error-prone or error-free damage avoidance pathway is taken. To understand the atomic details of this critical encounter, we have determined the crystal structures of the pol alpha family RB69 DNA polymerase with DNA containing the two most prevalent, spontaneously generated premutagenic lesions, an abasic site and 2'-deoxy-7,8-dihydro-8-oxoguanosine (8-oxodG). Identification of the interactions between these damaged nucleotides and the active site provides insight into the capacity of the polymerase to incorporate a base opposite the lesion. A novel open, catalytically inactive conformation of the DNA polymerase has been identified in the complex with a primed abasic site template. This structure provides the first molecular characterization of the DNA synthesis barrier caused by an abasic site and suggests a general mechanism for polymerase fidelity. In contrast, the structure of the ternary 8-oxodG:dCTP complex is almost identical to the replicating complex containing unmodified DNA, explaining the relative ease and fidelity by which this lesion is bypassed.
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(as it appears on PubMed at http://www.pubmed.gov), where 15057282 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15057282}}
==About this Structure==
==About this Structure==
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[[Category: Miller, H.]]
[[Category: Miller, H.]]
[[Category: Protein_dna complex]]
[[Category: Protein_dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 13 15:17:24 2008''

Revision as of 12:17, 13 July 2008

Image:1q9y.png

Template:STRUCTURE 1q9y

CRYSTAL STRUCTURE OF ENTEROBACTERIA PHAGE RB69 GP43 DNA POLYMERASE COMPLEXED WITH 8-OXOGUANOSINE CONTAINING DNA

Template:ABSTRACT PUBMED 15057282

About this Structure

1Q9Y is a Single protein structure of sequence from Enterobacteria phage rb69. Full crystallographic information is available from OCA.

Reference

Lesion (in)tolerance reveals insights into DNA replication fidelity., Freisinger E, Grollman AP, Miller H, Kisker C, EMBO J. 2004 Apr 7;23(7):1494-505. Epub 2004 Apr 1. PMID:15057282

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