This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1jb1
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1jb1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jb1, resolution 2.8Å" /> '''Lactobacillus casei H...)
Next diff →
Revision as of 15:56, 20 November 2007
|
Lactobacillus casei HprK/P Bound to Phosphate
Overview
HPr kinase/phosphatase (HprK/P) is a key regulatory enzyme controlling, carbon metabolism in Gram- positive bacteria. It catalyses the, ATP-dependent phosphorylation of Ser46 in HPr, a protein of the, phosphotransferase system, and also its dephosphorylation. HprK/P is, unrelated to eukaryotic protein kinases, but contains the Walker motif A, characteristic of nucleotide-binding proteins. We report here the X-ray, structure of an active fragment of Lactobacillus casei HprK/P at 2.8 A, resolution, solved by the multiwavelength anomalous dispersion method on a, seleniated protein (PDB code 1jb1). The protein is a hexamer, with each, subunit containing an ATP-binding domain similar to nucleoside/nucleotide, kinases, and a putative HPr-binding domain unrelated to the, substrate-binding domains of other kinases. The Walker motif A forms a, typical P-loop which binds inorganic phosphate in the crystal. We modelled, ATP binding by comparison with adenylate kinase, and designed a tentative, model of the complex with HPr based on a docking simulation. The results, confirm that HprK/P represents a new family of protein kinases, first, identified in bacteria, but which may also have members in eukaryotes.
About this Structure
1JB1 is a Single protein structure of sequence from Lactobacillus casei with PO4 as ligand. Full crystallographic information is available from OCA.
Reference
X-ray structure of HPr kinase: a bacterial protein kinase with a P-loop nucleotide-binding domain., Fieulaine S, Morera S, Poncet S, Monedero V, Gueguen-Chaignon V, Galinier A, Janin J, Deutscher J, Nessler S, EMBO J. 2001 Aug 1;20(15):3917-27. PMID:11483495
Page seeded by OCA on Tue Nov 20 18:03:49 2007
