1jch

From Proteopedia

---

Revision as of 15:59, 20 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1jch, resolution 3.02Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of Colicin E3 in Complex with its Immunity Protein

Overview

Colicins kill E. coli by a process that involves binding to a surface, receptor, entering the cell, and, finally, intoxicating it. The lethal, action of colicin E3 is a specific cleavage in the ribosomal decoding A, site. The crystal structure of colicin E3, reported here in a binary, complex with its immunity protein (IP), reveals a Y-shaped molecule with, the receptor binding domain forming a 100 A long stalk and the two, globular heads of the translocation domain (T) and the catalytic domain, (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active, site, IP prevents access of the active site to the ribosome.

About this Structure

1JCH is a Protein complex structure of sequences from Escherichia coli with CIT and GOL as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of colicin E3: implications for cell entry and ribosome inactivation., Soelaiman S, Jakes K, Wu N, Li C, Shoham M, Mol Cell. 2001 Nov;8(5):1053-62. PMID:11741540

Page seeded by OCA on Tue Nov 20 18:06:25 2007