2bd8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2bd8.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2bd8.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2bd8| PDB=2bd8 | SCENE= }}
{{STRUCTURE_2bd8| PDB=2bd8 | SCENE= }}
-
'''Porcine pancreatic elastase complexed with beta-casomorphin-7 and Arg-Phe at pH 5.0 (50 min soak) and immersed in pH 9 buffer for 30 seconds'''
+
===Porcine pancreatic elastase complexed with beta-casomorphin-7 and Arg-Phe at pH 5.0 (50 min soak) and immersed in pH 9 buffer for 30 seconds===
-
==Overview==
+
<!--
-
Mass spectrometric screening reveals that an unmodified natural heptapeptide--human beta-casomorphin-7, an internal sequence of human beta-casein that possesses opioid-like activity--reacts with porcine pancreatic elastase to form an unusually stable acyl-enzyme complex at low pH. X-ray crystallographic analysis (to 1.9 A resolution) at pH 5 shows continuous electron density linking the C-terminal isoleucine of beta-casomorphin-7 to Ser 195 through an ester bond. The structure reveals a well defined water molecule (Wat 317), equidistant between the carbon of the ester carbonyl and N epsilon 2 of His 57. Deprotonation of Wat 317 will produce a hydroxide ion positioned to attack the ester carbonyl through the favoured Burgi-Dunitz trajectory.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16754679}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16754679 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16754679}}
==About this Structure==
==About this Structure==
-
2BD8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BD8 OCA].
+
2BD8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BD8 OCA].
==Reference==
==Reference==
 +
Structural analyses on intermediates in serine protease catalysis., Liu B, Schofield CJ, Wilmouth RC, J Biol Chem. 2006 Aug 18;281(33):24024-35. Epub 2006 Jun 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16754679 16754679]
 +
Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9187653 9187653]
Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9187653 9187653]
 +
 +
X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate., Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J, Nat Struct Biol. 2001 Aug;8(8):689-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11473259 11473259]
[[Category: Pancreatic elastase]]
[[Category: Pancreatic elastase]]
-
[[Category: Single protein]]
+
[[Category: Protein complex]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Liu, B.]]
[[Category: Liu, B.]]
[[Category: Schofield, C J.]]
[[Category: Schofield, C J.]]
[[Category: Wilmouth, R C.]]
[[Category: Wilmouth, R C.]]
 +
[[Category: Hydrolase]]
[[Category: Serine proteinase]]
[[Category: Serine proteinase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:08:01 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 10:11:33 2008''

Revision as of 07:11, 23 July 2008

Image:2bd8.png

Template:STRUCTURE 2bd8

Porcine pancreatic elastase complexed with beta-casomorphin-7 and Arg-Phe at pH 5.0 (50 min soak) and immersed in pH 9 buffer for 30 seconds

Template:ABSTRACT PUBMED 16754679

About this Structure

2BD8 is a Protein complex structure of sequences from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Structural analyses on intermediates in serine protease catalysis., Liu B, Schofield CJ, Wilmouth RC, J Biol Chem. 2006 Aug 18;281(33):24024-35. Epub 2006 Jun 5. PMID:16754679

Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:9187653

X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate., Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J, Nat Struct Biol. 2001 Aug;8(8):689-94. PMID:11473259

Page seeded by OCA on Wed Jul 23 10:11:33 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bd8"
Personal tools