2bdb

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[[Image:2bdb.gif|left|200px]]
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{{STRUCTURE_2bdb| PDB=2bdb | SCENE= }}
{{STRUCTURE_2bdb| PDB=2bdb | SCENE= }}
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'''Porcine pancreatic elastase complexed with Asn-Pro-Ile and Ala-Ala at pH 5.0'''
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===Porcine pancreatic elastase complexed with Asn-Pro-Ile and Ala-Ala at pH 5.0===
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==Overview==
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Mass spectrometric screening reveals that an unmodified natural heptapeptide--human beta-casomorphin-7, an internal sequence of human beta-casein that possesses opioid-like activity--reacts with porcine pancreatic elastase to form an unusually stable acyl-enzyme complex at low pH. X-ray crystallographic analysis (to 1.9 A resolution) at pH 5 shows continuous electron density linking the C-terminal isoleucine of beta-casomorphin-7 to Ser 195 through an ester bond. The structure reveals a well defined water molecule (Wat 317), equidistant between the carbon of the ester carbonyl and N epsilon 2 of His 57. Deprotonation of Wat 317 will produce a hydroxide ion positioned to attack the ester carbonyl through the favoured Burgi-Dunitz trajectory.
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The line below this paragraph, {{ABSTRACT_PUBMED_16754679}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16754679 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16754679}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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Structural analyses on intermediates in serine protease catalysis., Liu B, Schofield CJ, Wilmouth RC, J Biol Chem. 2006 Aug 18;281(33):24024-35. Epub 2006 Jun 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16754679 16754679]
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Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9187653 9187653]
Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9187653 9187653]
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X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate., Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J, Nat Struct Biol. 2001 Aug;8(8):689-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11473259 11473259]
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X-ray structure of a serine protease acyl-enzyme complex at 0.95-A resolution., Katona G, Wilmouth RC, Wright PA, Berglund GI, Hajdu J, Neutze R, Schofield CJ, J Biol Chem. 2002 Jun 14;277(24):21962-70. Epub 2002 Mar 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11896054 11896054]
[[Category: Pancreatic elastase]]
[[Category: Pancreatic elastase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Schofield, C J.]]
[[Category: Schofield, C J.]]
[[Category: Wilmouth, R C.]]
[[Category: Wilmouth, R C.]]
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[[Category: Hydrolase]]
[[Category: Serine proteinase]]
[[Category: Serine proteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:08:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 10:11:46 2008''

Revision as of 07:11, 23 July 2008

Image:2bdb.png

Template:STRUCTURE 2bdb

Porcine pancreatic elastase complexed with Asn-Pro-Ile and Ala-Ala at pH 5.0

Template:ABSTRACT PUBMED 16754679

About this Structure

2BDB is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Structural analyses on intermediates in serine protease catalysis., Liu B, Schofield CJ, Wilmouth RC, J Biol Chem. 2006 Aug 18;281(33):24024-35. Epub 2006 Jun 5. PMID:16754679

Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:9187653

X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate., Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J, Nat Struct Biol. 2001 Aug;8(8):689-94. PMID:11473259

X-ray structure of a serine protease acyl-enzyme complex at 0.95-A resolution., Katona G, Wilmouth RC, Wright PA, Berglund GI, Hajdu J, Neutze R, Schofield CJ, J Biol Chem. 2002 Jun 14;277(24):21962-70. Epub 2002 Mar 14. PMID:11896054

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