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1je5
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(New page: 200px<br /><applet load="1je5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1je5, resolution 1.90Å" /> '''Crystal Structure of...)
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Revision as of 16:02, 20 November 2007
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Crystal Structure of gp2.5, a Single-Stranded DNA Binding Protein Encoded by Bacteriophage T7
Overview
The gene 2.5 protein (gp2.5) of bacteriophage T7 is a single-stranded DNA, (ssDNA) binding protein that has essential roles in DNA replication and, recombination. In addition to binding DNA, gp2.5 physically interacts with, T7 DNA polymerase and T7 primase-helicase during replication to coordinate, events at the replication fork. We have determined a 1.9-A crystal, structure of gp2.5 and show that it has a conserved OB-fold, (oligosaccharide/oligonucleotide binding fold) that is well adapted for, interactions with ssDNA. Superposition of the OB-folds of gp2.5 and other, ssDNA binding proteins reveals a conserved patch of aromatic residues that, stack against the bases of ssDNA in the other crystal structures, suggesting that gp2.5 binds to ssDNA in a similar manner. An acidic, C-terminal extension of the gp2.5 protein, which is required for dimer, formation and for interactions with the T7 DNA polymerase and the, primase-helicase, appears to be flexible and may act as a switch that, modulates the DNA binding affinity of gp2.5.
About this Structure
1JE5 is a Single protein structure of sequence from Bacteriophage t7 with CA as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the gene 2.5 protein, a single-stranded DNA binding protein encoded by bacteriophage T7., Hollis T, Stattel JM, Walther DS, Richardson CC, Ellenberger T, Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9557-62. Epub 2001 Jul 31. PMID:11481454
Page seeded by OCA on Tue Nov 20 18:09:22 2007
