1jf9

From Proteopedia

---

Revision as of 16:04, 20 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1jf9, resolution 2.Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of selenocysteine lyase

Overview

The Escherichia coli NifS CsdB protein is a member of the homodimeric, pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes, are capable of decomposing cysteine or selenocysteine into L-alanine and, sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity, for L-selenocysteine in comparison to l-cysteine, suggesting a role for, this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli, NifS CsdB reveals a high-resolution view of the active site of this enzyme, in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and, perselenide intermediates during catalysis, a necessary intermediate in, the formation of sulfur and selenium containing metabolites.

About this Structure

1JF9 is a Single protein structure of sequence from Escherichia coli with PLP as ligand. Active as Selenocysteine lyase, with EC number 4.4.1.16 Full crystallographic information is available from OCA.

Reference

Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:11827487

Page seeded by OCA on Tue Nov 20 18:11:16 2007