1jgg
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(New page: 200px<br /><applet load="1jgg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jgg, resolution 2.Å" /> '''Even-skipped Homeodoma...)
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Revision as of 16:06, 20 November 2007
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Even-skipped Homeodomain Complexed to AT-rich DNA
Overview
even-skipped is a homeobox gene important in controlling segment, patterning in the embryonic fruit fly. Its homeobox encodes a DNA binding, domain which binds with similar affinities to two DNA consensus sequences, one AT-rich, the other GC-rich. We describe a crystallographic analysis of, the Even-skipped homeodomain complexed to an AT-rich oligonucleotide at, 2.0 A resolution. The structure reveals a novel arrangement of two, homeodomains bound to one 10 bp DNA sequence in a tandem fashion. This, arrangement suggests a mechanism for the homeoproteins' regulatory, specificity. In addition, the functionally important residue Gln50 is, observed in multiple conformations making direct and water-mediated, hydrogen bonds with the DNA bases.
About this Structure
1JGG is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structure of the even-skipped homeodomain complexed to AT-rich DNA: new perspectives on homeodomain specificity., Hirsch JA, Aggarwal AK, EMBO J. 1995 Dec 15;14(24):6280-91. PMID:8557047
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