This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1jjh
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1jjh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jjh, resolution 2.50Å" /> '''E2 DNA-binding Domai...)
Next diff →
Revision as of 16:12, 20 November 2007
|
E2 DNA-binding Domain from Bovine Papillomavirus Type 1
Overview
The 2.5 A crystal structures of the DNA-binding domain of the E2 protein, from bovine papillomavirus strain 1 and its complex with DNA are, presented. E2 is a transcriptional regulatory protein that is also, involved in viral DNA replication. It is the structural prototype for a, novel class of DNA-binding proteins: dimeric beta-barrels with surface, alpha-helices that serve as recognition helices. These helices contain the, amino-acid residues involved in sequence-specifying interactions. The E2, proteins from different papillomavirus strains recognize and bind to the, same consensus 12 base-pair DNA sequence. However, recent evidence from, solution studies points to differences in the mechanisms by which E2 from, the related viral strains bovine papillomavirus-1 and human, papillomavirus-16 discriminate between DNA targets based on non-contacted, nucleotide sequences. This report provides evidence that sequence-specific, DNA-binding is accompanied by a rearrangement of protein subunits and, deformation of the DNA. These results suggest that, along with DNA, sequence-dependent conformational properties, protein subunit orientation, plays a significant role in the mechanisms of target selection utilized by, E2.
About this Structure
1JJH is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.
Reference
Subunit rearrangement accompanies sequence-specific DNA binding by the bovine papillomavirus-1 E2 protein., Hegde RS, Wang AF, Kim SS, Schapira M, J Mol Biol. 1998 Mar 6;276(4):797-808. PMID:9500927
Page seeded by OCA on Tue Nov 20 18:19:34 2007
