1jo5
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(New page: 200px<br /><applet load="1jo5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jo5" /> '''Rhodobacter sphaeroides Light Harvesting 1 b...)
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Revision as of 16:17, 20 November 2007
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Rhodobacter sphaeroides Light Harvesting 1 beta Subunit in Detergent Micelles
Overview
The light harvesting 1 antenna (LH1) complex from Rhodobacter sphaeroides, funnels excitation energy to the photosynthetic reaction center. Our, ultimate goal is to build up the structure of LH1 from structures of its, individual subunits, much as the antenna can self-assemble from its, components in membrane-mimicking detergent micelles. The beta subunit, adopts a nativelike conformation in Zwittergent 3:12 micelles as, demonstrated by its ability to take the first step of assembly, binding, BChl a. Multidimensional NMR spectroscopy shows that the beta subunit, folds as a helix((L12-S25))-hinge((G26-W28))-helix((L29-W44)) structure, with the helical regions for the 10 lowest-energy structures having, backbone rmsds of 0.26 and 0.24 A, respectively. Mn(2+) relaxation data, and the protein-detergent NOE pattern show the C-terminal helix embedded, in the micelle and the N-terminal helix lying along the detergent micelle, surface with a 60 degrees angle between their long axes. (15)N relaxation, data for residues L12-W44 are typical of a well-ordered protein with a, correlation time of 8.25 +/- 2.1 ns. The presence of the hinge region, placing the N-terminal helix along the membrane surface may be the, structural feature responsible for the functional differences observed, between the LH1 and LH2 beta subunits.
About this Structure
1JO5 is a Single protein structure of sequence from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.
Reference
Structure of the Rhodobacter sphaeroides light-harvesting 1 beta subunit in detergent micelles., Sorgen PL, Cahill SM, Krueger-Koplin RD, Krueger-Koplin ST, Schenck CC, Girvin ME, Biochemistry. 2002 Jan 8;41(1):31-41. PMID:11772000
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