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1pc8

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[[Image:1pc8.gif|left|200px]]
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{{STRUCTURE_1pc8| PDB=1pc8 | SCENE= }}
{{STRUCTURE_1pc8| PDB=1pc8 | SCENE= }}
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'''Crystal Structure of a novel form of mistletoe lectin from Himalayan Viscum album L. at 3.8A resolution'''
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===Crystal Structure of a novel form of mistletoe lectin from Himalayan Viscum album L. at 3.8A resolution===
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==Overview==
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This is the first report of the structural studies of a novel ribosome-inactivating protein (RIP) obtained from the Himalayan mistletoe (Viscum album) (HmRip). HmRip is a type II heterodimeric protein consisting of a toxic enzyme (A-chain) with an active site for ribosome inactivation and a lectin subunit (B-chain) with well defined sugar-binding sites. The crystal structure of HmRip has been determined at 3.8 A resolution and refined to a crystallographic R factor of 0.228 (R(free) = 0.271). A comparison of this structure with other type II RIPs reveals the presence of distinct structural features in the active site of the A-chain and in the 2gamma sugar-binding site of the B-chain. The conformation of the side chain of Tyr110, which is a conserved active-site residue in the A subunit, is strikingly different from those observed in other mistletoe RIPs, indicating its unique substrate-binding preference. The deletion of two important residues from the kink region after Ala231 in the 2gamma subdomain of the B-chain results in a significantly different conformation of the sugar-binding pocket. A ribosome-recognition site has also been identified in HmRip. The site is a shallow cavity, with the conserved residues Arg51, Asp70, Thr72 and Asn73 involved in the binding. The conformations of the antigenic epitopes of residues 1-20, 85-103 and 206-223 differ from those observed in other type II RIPs, resulting in the distinct antigenicity and pharmacological properties of HmRip.
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{{ABSTRACT_PUBMED_15583377}}
==About this Structure==
==About this Structure==
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[[Category: Mistletoe lectin]]
[[Category: Mistletoe lectin]]
[[Category: Novel form]]
[[Category: Novel form]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:25:10 2008''

Revision as of 09:25, 27 July 2008

Image:1pc8.png

Template:STRUCTURE 1pc8

Crystal Structure of a novel form of mistletoe lectin from Himalayan Viscum album L. at 3.8A resolution

Template:ABSTRACT PUBMED 15583377

About this Structure

1PC8 is a Protein complex structure of sequences from Viscum album. Full crystallographic information is available from OCA.

Reference

Structure of a novel ribosome-inactivating protein from a hemi-parasitic plant inhabiting the northwestern Himalayas., Mishra V, Ethayathulla AS, Sharma RS, Yadav S, Krauspenhaar R, Betzel C, Babu CR, Singh TP, Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 2):2295-304., Epub 2004 Nov 26. PMID:15583377

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