1oo4

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[[Image:1oo4.gif|left|200px]]
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{{Seed}}
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[[Image:1oo4.png|left|200px]]
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{{STRUCTURE_1oo4| PDB=1oo4 | SCENE= }}
{{STRUCTURE_1oo4| PDB=1oo4 | SCENE= }}
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'''P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase complexed to a peptide derived from PDGFr'''
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===P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase complexed to a peptide derived from PDGFr===
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==Overview==
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Understanding the specificity of Src homology 2 (SH2) domains is important because of their critical role in cell signaling. Previous genetic analysis has characterized mutants of the N-terminal src homology 2 (SH2) domain of the p85 subunit of phosphoinositide 3-kinase (PI3K). The P395S mutant exhibits a specificity for phosphopeptide binding different from that of the wild-type SH2. The P395S mutant has an increased affinity for the platelet-derived growth factor receptor (PDGFr) compared to polyomavirus middle T antigen (MT). Solution structures of the P395S mutant of the p85 N-SH2 alone and complexed to a PDGFr phosphopeptide were determined to explain the change in specificity. Chemical shift perturbations caused by different peptides were compared for mutant and wild-type structures. The results show that the single P395S mutation has broad effects on the structure. Furthermore, they provide a rationale for the observed changes in binding preference.
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The line below this paragraph, {{ABSTRACT_PUBMED_14503862}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 14503862 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14503862}}
==About this Structure==
==About this Structure==
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1OO4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OO4 OCA].
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1OO4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OO4 OCA].
==Reference==
==Reference==
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[[Category: Pdgfr complex]]
[[Category: Pdgfr complex]]
[[Category: Src homology 2 domain p85 regulatory subunit mutant]]
[[Category: Src homology 2 domain p85 regulatory subunit mutant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:05:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:26:58 2008''

Revision as of 09:26, 27 July 2008

Image:1oo4.png

Template:STRUCTURE 1oo4

P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase complexed to a peptide derived from PDGFr

Template:ABSTRACT PUBMED 14503862

About this Structure

1OO4 is a Single protein structure of sequence from Bos taurus. Full experimental information is available from OCA.

Reference

Nuclear magnetic resonance structure of the P395S mutant of the N-SH2 domain of the p85 subunit of PI3 kinase: an SH2 domain with altered specificity., Gunther UL, Weyrauch B, Zhang X, Schaffhausen B, Biochemistry. 2003 Sep 30;42(38):11120-7. PMID:14503862

Page seeded by OCA on Sun Jul 27 12:26:58 2008

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