2sam

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2sam.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2sam.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2sam| PDB=2sam | SCENE= }}
{{STRUCTURE_2sam| PDB=2sam | SCENE= }}
-
'''STRUCTURE OF THE PROTEASE FROM SIMIAN IMMUNODEFICIENCY VIRUS: COMPLEX WITH AN IRREVERSIBLE NON-PEPTIDE INHIBITOR'''
+
===STRUCTURE OF THE PROTEASE FROM SIMIAN IMMUNODEFICIENCY VIRUS: COMPLEX WITH AN IRREVERSIBLE NON-PEPTIDE INHIBITOR===
-
==Overview==
+
<!--
-
A variant of the simian immunodeficiency virus protease (SIV PR), covalently bound to the inhibitor 1,2-epoxy-3-(p-nitrophenoxy)propane (EPNP), was crystallized. The structure of the inhibited complex was determined by X-ray crystallography to a resolution of 2.4 A and refined to an R factor of 19%. The variant, SIV PR S4H, was shown to diminish the rate of autolysis by at least 4-fold without affecting enzymatic parameters. The overall root mean square (rms) deviation of the alpha-carbons from the structure of HIV-1PR complexed with a peptidomimetic inhibitor (7HVP) was 1.16 A. The major differences are concentrated in three surface loops with rms differences between 1.2 and 2.1 A. For 60% of the molecule the rms deviation was only 0.6 A. The structure reveals one molecule of EPNP bound per protease dimer, a stoichiometry confirmed by mass spectral analysis. The epoxide moiety forms a covalent bond with either of the active site aspartic acids of the dimer, and the phenyl moiety occupies the P1 binding site. The EPNP nitro group interacts with Arg 8. This structure suggests a starting template for the design of nonpeptide-based irreversible inhibitors of the SIV and related HIV-1 and HIV-2 PRs.
+
The line below this paragraph, {{ABSTRACT_PUBMED_8241141}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 8241141 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_8241141}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Salto, R.]]
[[Category: Salto, R.]]
[[Category: Stroud, R M.]]
[[Category: Stroud, R M.]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:17:06 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:29:38 2008''

Revision as of 09:29, 27 July 2008

Image:2sam.png

Template:STRUCTURE 2sam

STRUCTURE OF THE PROTEASE FROM SIMIAN IMMUNODEFICIENCY VIRUS: COMPLEX WITH AN IRREVERSIBLE NON-PEPTIDE INHIBITOR

Template:ABSTRACT PUBMED 8241141

About this Structure

2SAM is a Single protein structure of sequence from Simian immunodeficiency virus. Full crystallographic information is available from OCA.

Reference

Structure of the protease from simian immunodeficiency virus: complex with an irreversible nonpeptide inhibitor., Rose RB, Rose JR, Salto R, Craik CS, Stroud RM, Biochemistry. 1993 Nov 23;32(46):12498-507. PMID:8241141

Page seeded by OCA on Sun Jul 27 12:29:38 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2sam"
Personal tools