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1p1a

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{{STRUCTURE_1p1a| PDB=1p1a | SCENE= }}
{{STRUCTURE_1p1a| PDB=1p1a | SCENE= }}
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'''NMR structure of ubiquitin-like domain of hHR23B'''
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===NMR structure of ubiquitin-like domain of hHR23B===
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==Overview==
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hHR23B is the human homologue of the yeast protein RAD23 and is known to participate in DNA repair by stabilizing xeroderma pigmentosum group C protein. However, hHR23B and RAD23 also have many important functions related to general proteolysis. hHR23B consists of N-terminal ubiquitin-like (UbL), ubiquitin association 1 (UBA1), xeroderma pigmentosum group C binding, and UBA2 domains. The UBA domains interact with ubiquitin (Ub) and inhibit the assembly of polyubiquitin. On the other hand, the UbL domain interacts with the poly-Ub binding site 2 (PUbS2) domain of the S5a protein, which can carry polyubiquitinated substrates into the proteasome. We calculated the NMR structure of the UbL domain of hHR23B and determined binding surfaces of UbL and Ub to UBA1, UBA2, of hHR23B and PUbS2 of S5a by using chemical shift perturbation. Interestingly, the surfaces of UbL and Ub that bind to UBA1, UBA2, and PUbS2 are similar, consisting of five beta-strands and their connecting loops. This is the first report that an intramolecular interaction between UbL and UBA domains is possible, and this interaction could be important for the control of proteolysis by hHR23B. The binding specificities of UbL and Ub for PUbS1, PUbS2, and general ubiquitin-interacting motifs, which share the LALA motif, were evaluated. The UBA domains bind to the surface of Ub including Lys-48, which is required for multiubiquitin assembly, possibly explaining the observed inhibition of multiubiquitination by hHR23B. The UBA domains bind to UbL through electrostatic interactions supported by hydrophobic interactions and to Ub mainly through hydrophobic interactions supported by electrostatic interactions.
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{{ABSTRACT_PUBMED_12832454}}
==About this Structure==
==About this Structure==
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1P1A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P1A OCA].
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1P1A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P1A OCA].
==Reference==
==Reference==
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[[Category: Ryu, K S.]]
[[Category: Ryu, K S.]]
[[Category: Ubiquitin-like]]
[[Category: Ubiquitin-like]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:31:16 2008''

Revision as of 09:31, 27 July 2008

Image:1p1a.png

Template:STRUCTURE 1p1a

NMR structure of ubiquitin-like domain of hHR23B

Template:ABSTRACT PUBMED 12832454

About this Structure

1P1A is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Binding surface mapping of intra- and interdomain interactions among hHR23B, ubiquitin, and polyubiquitin binding site 2 of S5a., Ryu KS, Lee KJ, Bae SH, Kim BK, Kim KA, Choi BS, J Biol Chem. 2003 Sep 19;278(38):36621-7. Epub 2003 Jun 28. PMID:12832454

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