1sax

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{{STRUCTURE_1sax| PDB=1sax | SCENE= }}
{{STRUCTURE_1sax| PDB=1sax | SCENE= }}
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'''Three-dimensional structure of s.aureus methicillin-resistance regulating transcriptional repressor meci in complex with 25-bp ds-DNA'''
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===Three-dimensional structure of s.aureus methicillin-resistance regulating transcriptional repressor meci in complex with 25-bp ds-DNA===
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==Overview==
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Bacterial resistance to antibiotics poses a serious worldwide public health problem due to the high morbidity and mortality caused by infectious diseases. Most hospital-onset infections are associated with methicillin-resistant Staphylococcus aureus (MRSA) strains that have acquired multiple drug resistance to beta-lactam antibiotics. In a response to antimicrobial stress, nearly all clinical MRSA isolates produce beta-lactamase (BlaZ) and a penicillin-binding protein with low affinity for beta-lactam antibiotics (PBP2a, also known as PBP2' or MecA). Both effectors are regulated by homologous signal transduction systems consisting of a sensor/transducer and a transcriptional repressor. MecI (methicillin repressor) blocks mecA but also blaZ transcription and that of itself and the co-transcribed sensor/transducer. The structure of MecI in complex with a cognate operator double-stranded DNA reveals a homodimeric arrangement with a novel C-terminal spiral staircase dimerization domain responsible for dimer integrity. Each protomer interacts with the DNA major groove through a winged helix DNA-binding domain and specifically recognizes the nucleotide sequence 5'-Gua-Thy-Ade-X-Thy-3'. This results in an unusual convex bending of the DNA helix. The structure of this first molecular determinant of methicillin resistance in complex with its target DNA provides insights into its regulatory mechanism and paves the way for new antimicrobial strategies against MRSA.
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{{ABSTRACT_PUBMED_14960592}}
==About this Structure==
==About this Structure==
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[[Category: Potempa, J.]]
[[Category: Potempa, J.]]
[[Category: Winged helix-turn-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:33:22 2008''

Revision as of 09:33, 27 July 2008

Image:1sax.png

Template:STRUCTURE 1sax

Three-dimensional structure of s.aureus methicillin-resistance regulating transcriptional repressor meci in complex with 25-bp ds-DNA

Template:ABSTRACT PUBMED 14960592

About this Structure

1SAX is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

On the transcriptional regulation of methicillin resistance: MecI repressor in complex with its operator., Garcia-Castellanos R, Mallorqui-Fernandez G, Marrero A, Potempa J, Coll M, Gomis-Ruth FX, J Biol Chem. 2004 Apr 23;279(17):17888-96. Epub 2004 Feb 11. PMID:14960592

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