2d38

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[[Image:2d38.gif|left|200px]]
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{{STRUCTURE_2d38| PDB=2d38 | SCENE= }}
{{STRUCTURE_2d38| PDB=2d38 | SCENE= }}
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'''The Crystal Structure of Flavin Reductase HpaC complexed with NADP+'''
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===The Crystal Structure of Flavin Reductase HpaC complexed with NADP+===
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==Overview==
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4-Hydroxyphenylacetate (4-HPA) is oxidized as an energy source by two component enzymes, the large component (HpaB) and the small component (HpaC). HpaB is a 4-HPA monooxygenase that utilizes FADH(2) supplied by a flavin reductase HpaC. We determined the crystal structure of HpaC (ST0723) from the aerobic thermoacidophilic crenarchaeon Sulfolobus tokodaii strain 7 in its three states [NAD(P)(+)-free, NAD(+)-bound, and NADP(+)-bound]. HpaC exists as a homodimer, and each monomer was found to contain an FMN. HpaC preferred FMN to FAD because there was not enough space to accommodate the AMP moiety of FAD in its flavin-binding site. The most striking difference between the NAD(P)(+)-free and the NAD(+)/NADP(+)-bound structures was observed in the N-terminal helix. The N-terminal helices in the NAD(+)/NADP(+)-bound structures rotated ca. 20 degrees relative to the NAD(P)(+)-free structure. The bound NAD(+) has a compact folded conformation with nearly parallel stacking rings of nicotinamide and adenine. The nicotinamide of NAD(+) stacked the isoalloxazine ring of FMN so that NADH could directly transfer hydride. The bound NADP(+) also had a compact conformation but was bound in a reverse direction, which was not suitable for hydride transfer.
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(as it appears on PubMed at http://www.pubmed.gov), where 16618099 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16618099}}
==About this Structure==
==About this Structure==
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[[Category: Tanokura, M.]]
[[Category: Tanokura, M.]]
[[Category: Flavin reductase]]
[[Category: Flavin reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:37:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:44:33 2008''

Revision as of 09:44, 27 July 2008

Image:2d38.png

Template:STRUCTURE 2d38

The Crystal Structure of Flavin Reductase HpaC complexed with NADP+

Template:ABSTRACT PUBMED 16618099

About this Structure

2D38 is a Single protein structure of sequence from Sulfolobus tokodaii str. 7. Full crystallographic information is available from OCA.

Reference

Crystal structures of the short-chain flavin reductase HpaC from Sulfolobus tokodaii strain 7 in its three states: NAD(P)(+)(-)free, NAD(+)(-)bound, and NADP(+)(-)bound., Okai M, Kudo N, Lee WC, Kamo M, Nagata K, Tanokura M, Biochemistry. 2006 Apr 25;45(16):5103-10. PMID:16618099

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