This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2jt7

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2jt7.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2jt7.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2jt7| PDB=2jt7 | SCENE= }}
{{STRUCTURE_2jt7| PDB=2jt7 | SCENE= }}
-
'''NMR solution structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex'''
+
===NMR solution structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex===
-
==Overview==
+
<!--
-
The complex between distamycin A and the parallel DNA quadruplex [d(TGGGGT)]4 has been studied by 1H NMR spectroscopy and isothermal titration calorimetry (ITC). To unambiguously assert that distamycin A interacts with the grooves of the quadruplex [d(TGGGGT)]4, we have analyzed the NMR titration profile of a modified quadruplex, namely [d(TGGMeGGT)]4, and we have applied the recently developed differential frequency-saturation transfer difference (DF-STD) method, for assessing the ligand-DNA binding mode. The three-dimensional structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex has been determined by an in-depth NMR study followed by dynamics and mechanics calculations. All results unequivocally indicate that distamycin molecules interact with [d(TGGGGT)]4 in a 4:1 binding mode, with two antiparallel distamycin dimers that bind simultaneously two opposite grooves of the quadruplex. The affinity between distamycin A and [d(TGGGGT)]4 enhances ( approximately 10-fold) when the ratio of distamycin A to the quadruplex is increased. In this paper we report the first three-dimensional structure of a groove-binder molecule complexed to a DNA quadruplex structure.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18052170}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18052170 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18052170}}
==About this Structure==
==About this Structure==
-
2JT7 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JT7 OCA].
+
2JT7 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JT7 OCA].
==Reference==
==Reference==
Line 29: Line 33:
[[Category: Telomere]]
[[Category: Telomere]]
[[Category: Tggggt]]
[[Category: Tggggt]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:16:24 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:57:01 2008''

Revision as of 09:57, 27 July 2008

Image:2jt7.png

Template:STRUCTURE 2jt7

NMR solution structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex

Template:ABSTRACT PUBMED 18052170

About this Structure

2JT7 is a Single protein structure. Full experimental information is available from OCA.

Reference

Structural and thermodynamic studies of the interaction of distamycin A with the parallel quadruplex structure [d(TGGGGT)]4., Martino L, Virno A, Pagano B, Virgilio A, Di Micco S, Galeone A, Giancola C, Bifulco G, Mayol L, Randazzo A, J Am Chem Soc. 2007 Dec 26;129(51):16048-56. Epub 2007 Dec 4. PMID:18052170

Page seeded by OCA on Sun Jul 27 12:57:01 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jt7"
Personal tools