1jr3
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(New page: 200px<br /><applet load="1jr3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jr3, resolution 2.7Å" /> '''Crystal Structure of ...)
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Revision as of 16:23, 20 November 2007
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Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III
Overview
The gamma complex, an AAA+ ATPase, is the bacterial homolog of eukaryotic, replication factor C (RFC) that loads the sliding clamp (beta, homologous, to PCNA) onto DNA. The 2.7/3.0 A crystal structure of gamma complex, reveals a pentameric arrangement of subunits, with stoichiometry, delta':gamma(3):delta. The C-terminal domains of the subunits form a, circular collar that supports an asymmetric arrangement of the N-terminal, ATP binding domains of the gamma motor and the structurally related, domains of the delta' stator and the delta wrench. The structure suggests, a mechanism by which the gamma complex switches between a closed state, in, which the beta-interacting element of delta is hidden by delta', and an, open form similar to the crystal structure, in which delta is free to bind, to beta.
About this Structure
1JR3 is a Protein complex structure of sequences from Escherichia coli with ZN and SO4 as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Crystal structure of the processivity clamp loader gamma (gamma) complex of E. coli DNA polymerase III., Jeruzalmi D, O'Donnell M, Kuriyan J, Cell. 2001 Aug 24;106(4):429-41. PMID:11525729
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