2q10

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{{STRUCTURE_2q10| PDB=2q10 | SCENE= }}
{{STRUCTURE_2q10| PDB=2q10 | SCENE= }}
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'''RESTRICTION ENDONUCLEASE BcnI (WILD TYPE)-COGNATE DNA SUBSTRATE COMPLEX'''
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===RESTRICTION ENDONUCLEASE BcnI (WILD TYPE)-COGNATE DNA SUBSTRATE COMPLEX===
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==Overview==
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Restriction endonuclease BcnI cleaves duplex DNA containing the sequence CC/SGG (S stands for C or G, / designates a cleavage position) to generate staggered products with single nucleotide 5'-overhangs. Here, we show that BcnI functions as a monomer that interacts with its target DNA in 1:1 molar ratio and report crystal structures of BcnI in the absence and in the presence of DNA. In the complex with DNA, BcnI makes specific contacts with all five bases of the target sequence and not just with a half-site, as the protomer of a typical dimeric restriction endonuclease. Our data are inconsistent with BcnI dimerization and suggest that the enzyme introduces double-strand breaks by sequentially nicking individual DNA strands, although this remains to be confirmed by kinetic experiments. BcnI is remotely similar to the DNA repair protein MutH and shares approximately 20% sequence identity with the restriction endonuclease MvaI, which is specific for the related sequence CC/WGG (W stands for A or T). As expected, BcnI is structurally similar to MvaI and recognizes conserved bases in the target sequence similarly but not identically. BcnI has a unique machinery for the recognition of the central base-pair.
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{{ABSTRACT_PUBMED_17445830}}
==About this Structure==
==About this Structure==
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[[Category: Endonuclease-dna complex]]
[[Category: Endonuclease-dna complex]]
[[Category: Restriction enzyme]]
[[Category: Restriction enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:00:53 2008''

Revision as of 10:00, 27 July 2008

Image:2q10.png

Template:STRUCTURE 2q10

RESTRICTION ENDONUCLEASE BcnI (WILD TYPE)-COGNATE DNA SUBSTRATE COMPLEX

Template:ABSTRACT PUBMED 17445830

About this Structure

2Q10 is a Single protein structure of sequence from Brevibacillus centrosporus. Full crystallographic information is available from OCA.

Reference

Monomeric restriction endonuclease BcnI in the apo form and in an asymmetric complex with target DNA., Sokolowska M, Kaus-Drobek M, Czapinska H, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M, J Mol Biol. 2007 Jun 8;369(3):722-34. Epub 2007 Mar 15. PMID:17445830

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