2fmj

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[[Image:2fmj.gif|left|200px]]
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{{STRUCTURE_2fmj| PDB=2fmj | SCENE= }}
{{STRUCTURE_2fmj| PDB=2fmj | SCENE= }}
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'''220-loop mutant of streptomyces griseus trypsin'''
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===220-loop mutant of streptomyces griseus trypsin===
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==Overview==
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Serine proteases of the chymotrypsin family show a dichotomous amino acid distribution for residue 225. Enzymes carrying Tyr at position 225 are activated by Na(+), whereas those carrying Pro are devoid of Na(+) binding and activation. Previous studies have demonstrated that the Y225P conversion is sufficient to abrogate Na(+) activation in several enzymes. However, the reverse substitution P225Y is necessary but not sufficient to introduce Na(+) binding and activation. Here we report that Streptomyces griseus trypsin, carrying Pro-225, can be engineered into a Na(+)-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa. The findings represent the first instance of an engineered Na(+)-activated enzyme and a proof of principle that should enable the design of other proteases with enhanced catalytic activity and allosteric regulation mediated by monovalent cation binding.
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(as it appears on PubMed at http://www.pubmed.gov), where 16503653 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16503653}}
==About this Structure==
==About this Structure==
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[[Category: Serine protease]]
[[Category: Serine protease]]
[[Category: Trypsin]]
[[Category: Trypsin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:04:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:01:18 2008''

Revision as of 10:01, 27 July 2008

Image:2fmj.png

Template:STRUCTURE 2fmj

220-loop mutant of streptomyces griseus trypsin

Template:ABSTRACT PUBMED 16503653

About this Structure

2FMJ is a Single protein structure of sequence from Streptomyces griseus. Full crystallographic information is available from OCA.

Reference

Conversion of trypsin into a Na(+)-activated enzyme., Page MJ, Bleackley MR, Wong S, MacGillivray RT, Di Cera E, Biochemistry. 2006 Mar 7;45(9):2987-93. PMID:16503653

Page seeded by OCA on Sun Jul 27 13:01:18 2008

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