1x2h

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{{STRUCTURE_1x2h| PDB=1x2h | SCENE= }}
{{STRUCTURE_1x2h| PDB=1x2h | SCENE= }}
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'''Crystal Structure of Lipate-Protein Ligase A from Escherichia coli complexed with lipoic acid'''
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===Crystal Structure of Lipate-Protein Ligase A from Escherichia coli complexed with lipoic acid===
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==Overview==
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Lipoate-protein ligase A (LplA) catalyzes the formation of lipoyl-AMP from lipoate and ATP and then transfers the lipoyl moiety to a specific lysine residue on the acyltransferase subunit of alpha-ketoacid dehydrogenase complexes and on H-protein of the glycine cleavage system. The lypoyllysine arm plays a pivotal role in the complexes by shuttling the reaction intermediate and reducing equivalents between the active sites of the components of the complexes. We have determined the X-ray crystal structures of Escherichia coli LplA alone and in a complex with lipoic acid at 2.4 and 2.9 angstroms resolution, respectively. The structure of LplA consists of a large N-terminal domain and a small C-terminal domain. The structure identifies the substrate binding pocket at the interface between the two domains. Lipoic acid is bound in a hydrophobic cavity in the N-terminal domain through hydrophobic interactions and a weak hydrogen bond between carboxyl group of lipoic acid and the Ser-72 or Arg-140 residue of LplA. No large conformational change was observed in the main chain structure upon the binding of lipoic acid.
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(as it appears on PubMed at http://www.pubmed.gov), where 16043486 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16043486}}
==About this Structure==
==About this Structure==
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[[Category: Post-translational modification]]
[[Category: Post-translational modification]]
[[Category: Protein acylation]]
[[Category: Protein acylation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:26:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:02:49 2008''

Revision as of 10:02, 27 July 2008

Image:1x2h.png

Template:STRUCTURE 1x2h

Crystal Structure of Lipate-Protein Ligase A from Escherichia coli complexed with lipoic acid

Template:ABSTRACT PUBMED 16043486

About this Structure

1X2H is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of lipoate-protein ligase A from Escherichia coli. Determination of the lipoic acid-binding site., Fujiwara K, Toma S, Okamura-Ikeda K, Motokawa Y, Nakagawa A, Taniguchi H, J Biol Chem. 2005 Sep 30;280(39):33645-51. Epub 2005 Jul 25. PMID:16043486

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