2z9v

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{{STRUCTURE_2z9v| PDB=2z9v | SCENE= }}
{{STRUCTURE_2z9v| PDB=2z9v | SCENE= }}
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'''Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxamine'''
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===Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxamine===
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==Overview==
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Pyridoxamine-pyruvate aminotransferase (PPAT; EC 2.6.1.30) is a pyridoxal 5'-phosphate-independent aminotransferase and catalyzes reversible transamination between pyridoxamine and pyruvate to form pyridoxal and L-alanine. The crystal structure of PPAT from Mesorhizobium loti has been solved in space group P4(3)2(1)2 and was refined to an R factor of 15.6% (R(free) = 20.6%) at 2.0 A resolution. In addition, the structures of PPAT in complexes with pyridoxamine, pyridoxal, and pyridoxyl-L-alanine have been refined to R factors of 15.6, 15.4, and 14.5% (R(free) = 18.6, 18.1, and 18.4%) at 1.7, 1.7, and 2.0 A resolution, respectively. PPAT is a homotetramer and each subunit is composed of a large N-terminal domain, consisting of seven beta-sheets and eight alpha-helices, and a smaller C-terminal domain, consisting of three beta-sheets and four alpha-helices. The substrate pyridoxal is bound through an aldimine linkage to Lys-197 in the active site. The alpha-carboxylate group of the substrate amino/keto acid is hydrogen-bonded to Arg-336 and Arg-345. The structures revealed that the bulky side chain of Glu-68 interfered with the binding of the phosphate moiety of pyridoxal 5'-phosphate and made PPAT specific to pyridoxal. The reaction mechanism of the enzyme is discussed based on the structures and kinetics results.
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{{ABSTRACT_PUBMED_17989071}}
==About this Structure==
==About this Structure==
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[[Category: Pyridoxamine]]
[[Category: Pyridoxamine]]
[[Category: Pyruvate]]
[[Category: Pyruvate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:15:49 2008''

Revision as of 10:15, 27 July 2008

Image:2z9v.png

Template:STRUCTURE 2z9v

Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxamine

Template:ABSTRACT PUBMED 17989071

About this Structure

2Z9V is a Single protein structure of sequence from Mesorhizobium loti. Full crystallographic information is available from OCA.

Reference

Crystal structure of pyridoxamine-pyruvate aminotransferase from Mesorhizobium loti MAFF303099., Yoshikane Y, Yokochi N, Yamasaki M, Mizutani K, Ohnishi K, Mikami B, Hayashi H, Yagi T, J Biol Chem. 2008 Jan 11;283(2):1120-7. Epub 2007 Nov 6. PMID:17989071

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