2atg

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[[Image:2atg.gif|left|200px]]
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{{STRUCTURE_2atg| PDB=2atg | SCENE= }}
{{STRUCTURE_2atg| PDB=2atg | SCENE= }}
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'''NMR structure of Retrocyclin-2 in SDS'''
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===NMR structure of Retrocyclin-2 in SDS===
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==Overview==
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Retrocyclins are circular mini-defensins with significant potential as agents against human immunodeficiency virus, influenza A, and herpes simplex virus. Retrocyclins bind carbohydrate-containing surface molecules such as gp120 and CD4 with high affinity (Kd, 10-100 nM), promoting their localization on cell membranes. The structural features important for activity have yet to be fully elucidated, but here, we have determined the first three-dimensional structure of a retrocyclin, namely, one of the most potent forms, retrocyclin-2. In the presence of SDS micelles, a well-defined beta-hairpin braced by three disulfide bonds that defines the cystine ladder motif is present. By contrast, a well-defined structure could not be determined in aqueous solution, suggesting that the presence of SDS micelles stabilizes the extended conformation of retrocyclin-2. Translational diffusion measurements indicate that retrocyclin-2 interacts with the SDS micelles, and such a membrane-like interaction may be an important feature in the mechanism of action of these antimicrobial peptides. Analytical ultracentrifugation and the NMR data indicated that retrocyclin-2 self-associates to form a trimer in a concentration-dependent manner. The ability to self-associate may contribute to the high-affinity binding of retrocyclins for glycoproteins by increasing the valency and enhancing the ability of retrocyclins to cross-link cell surface glycoproteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 17685559 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17685559}}
==About this Structure==
==About this Structure==
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2ATG is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ATG OCA].
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2ATG is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ATG OCA].
==Reference==
==Reference==
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[[Category: Circular peptide]]
[[Category: Circular peptide]]
[[Category: Laddered disulfide connectivity]]
[[Category: Laddered disulfide connectivity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 28 09:27:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:19:08 2008''

Revision as of 10:19, 27 July 2008

Image:2atg.png

Template:STRUCTURE 2atg

NMR structure of Retrocyclin-2 in SDS

Template:ABSTRACT PUBMED 17685559

About this Structure

2ATG is a Single protein structure. Full experimental information is available from OCA.

Reference

Retrocyclin-2: structural analysis of a potent anti-HIV theta-defensin., Daly NL, Chen YK, Rosengren KJ, Marx UC, Phillips ML, Waring AJ, Wang W, Lehrer RI, Craik DJ, Biochemistry. 2007 Sep 4;46(35):9920-8. Epub 2007 Aug 8. PMID:17685559

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