1jt8
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(New page: 200px<br /><applet load="1jt8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jt8" /> '''ARCHAEAL INITIATION FACTOR-1A, AIF-1A'''<br ...)
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Revision as of 16:26, 20 November 2007
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ARCHAEAL INITIATION FACTOR-1A, AIF-1A
Overview
Translation initiation factor 1A (aIF-1A) from the archaeon Methanococcus, jannaschii was expressed in Escherichia coli, purified, and characterized, in terms of its structure and dynamics using multidimensional NMR methods., The protein was found to be a member of the OB-fold family of, RNA-associated proteins, containing a barrel of five beta-strands, a, feature that is shared with the homologous eukaryotic translation, initiation factor 1A (eIF-1A), as well as the prokaryotic translation, initiation factor IF1. External to the beta barrel, aIF-1A contains an, alpha-helix at its C-terminal and a flexible loop at its N-terminal, features that are qualitatively similar to those found in eIF-1A, but not, present in prokaryotic IF1. The structural model of aIF-1A, when used in, combination with primary sequence information for aIF-1A in divergent, species, permitted the most-conserved residues on the protein surface to, be identified, including the most likely candidates for direct interaction, with the 16S ribosomal RNA and other components of the translational, apparatus. Several of the conserved surface residues appear to be unique, to the archaea. Nitrogen-15 relaxation and amide exchange rate data were, used to characterize the internal motions within aIF-1A, providing, evidence that the protein surfaces that are most likely to participate in, intermolecular interactions are relatively flexible. A model is proposed, suggesting some specific interactions that may occur between aIF-1A and, the small subunit of the archaeal ribosome.
About this Structure
1JT8 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
Reference
Structure and dynamics of translation initiation factor aIF-1A from the archaeon Methanococcus jannaschii determined by NMR spectroscopy., Li W, Hoffman DW, Protein Sci. 2001 Dec;10(12):2426-38. PMID:11714910
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