2qry

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{{STRUCTURE_2qry| PDB=2qry | SCENE= }}
{{STRUCTURE_2qry| PDB=2qry | SCENE= }}
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'''Periplasmic thiamin binding protein'''
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===Periplasmic thiamin binding protein===
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==Overview==
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ATP-binding cassette (ABC) transporters are responsible for the transport of a wide variety of water-soluble molecules and ions into prokaryotic cells. In Gram-negative bacteria, periplasmic-binding proteins deliver ions or molecules such as thiamin to the membrane-bound ABC transporter. The gene for the thiamin-binding protein tbpA has been identified in both Escherichia coli and Salmonella typhimurium. Here we report the crystal structure of TbpA from E. coli with bound thiamin monophosphate. The structure was determined at 2.25 A resolution using single-wavelength anomalous diffraction experiments, despite the presence of nonmerohedral twinning. The crystal structure shows that TbpA belongs to the group II periplasmic-binding protein family. Equilibrium binding measurements showed similar dissociation constants for thiamin, thiamin monophosphate, and thiamin pyrophosphate. Analysis of the binding site by molecular modeling demonstrated how TbpA binds all three forms of thiamin. A comparison of TbpA and thiaminase-I, a thiamin-degrading enzyme, revealed structural similarity between the two proteins, especially in domain 1, suggesting that the two proteins evolved from a common ancestor.
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(as it appears on PubMed at http://www.pubmed.gov), where 18177053 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18177053}}
==About this Structure==
==About this Structure==
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[[Category: Thiamin binding protein]]
[[Category: Thiamin binding protein]]
[[Category: Transport protein]]
[[Category: Transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:33:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:21:24 2008''

Revision as of 10:21, 27 July 2008

Image:2qry.png

Template:STRUCTURE 2qry

Periplasmic thiamin binding protein

Template:ABSTRACT PUBMED 18177053

About this Structure

2QRY is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural Similarities between Thiamin-Binding Protein and Thiaminase-I Suggest a Common Ancestor(,)., Soriano EV, Rajashankar KR, Hanes JW, Bale S, Begley TP, Ealick SE, Biochemistry. 2008 Feb 5;47(5):1346-57. Epub 2008 Jan 5. PMID:18177053

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