2nwc

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{{STRUCTURE_2nwc| PDB=2nwc | SCENE= }}
{{STRUCTURE_2nwc| PDB=2nwc | SCENE= }}
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'''A 3.02 angstrom crystal structure of wild-type apo GroEL in a monoclinic space group'''
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===A 3.02 angstrom crystal structure of wild-type apo GroEL in a monoclinic space group===
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==Overview==
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GroEL is a member of the ATP-dependent chaperonin family that promotes the proper folding of many cytosolic bacterial proteins. The structures of GroEL in a variety of different states have been determined using X-ray crystallography and cryo-electron microscopy. In this study, a 3.02 A crystal structure of the native GroEL complex from Escherichia coli is presented. The complex was purified and crystallized in the absence of potassium ions, which allowed evaluation of the structural changes that may occur in response to cognate potassium-ion binding by comparison to the previously determined wild-type GroEL structure (PDB code 1xck), in which potassium ions were observed in all 14 subunits. In general, the structure is similar to the previously determined wild-type GroEL crystal structure with some differences in regard to temperature-factor distribution.
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(as it appears on PubMed at http://www.pubmed.gov), where 17554162 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17554162}}
==About this Structure==
==About this Structure==
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[[Category: Chaperonin]]
[[Category: Chaperonin]]
[[Category: Hsp60]]
[[Category: Hsp60]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:59:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:28:47 2008''

Revision as of 10:28, 27 July 2008

Image:2nwc.png

Template:STRUCTURE 2nwc

A 3.02 angstrom crystal structure of wild-type apo GroEL in a monoclinic space group

Template:ABSTRACT PUBMED 17554162

About this Structure

2NWC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Purification, crystallization and structure determination of native GroEL from Escherichia coli lacking bound potassium ions., Kiser PD, Lodowski DT, Palczewski K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt, 6):457-61. Epub 2007 May 5. PMID:17554162

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