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1sa9

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{{STRUCTURE_1sa9| PDB=1sa9 | SCENE= }}
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'''Crystal Structure of the RNA octamer GGCGAGCC'''
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===Crystal Structure of the RNA octamer GGCGAGCC===
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==Overview==
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The crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and 5'-GIC(GA)GCC-3', have been determined from X-ray diffraction data to 2.8 and 2.7 A resolution, respectively. The RNA octamers crystallize in isomorphous unit cells containing two mispairs arranged in a self-complementary manner and one single strand in the asymmetric unit. The single strand pairs with another single strand related by crystallographic symmetry to form a third unique double helix. Tandem non-Watson-Crick G.A/A.G base pairs of the sheared type comprise an internal loop in the middle of each duplex. The NMR structure of this octameric RNA sequence is also known, allowing comparison of the variation between the six crystallographic duplexes and the solution structure. In the symmetric duplex of the octamer containing inosine, the sheared G.A pairs incorporate a bound water molecule. This duplex also binds one water molecule per strand in the minor groove adjacent to the G.A pairs.
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{{ABSTRACT_PUBMED_15103128}}
==About this Structure==
==About this Structure==
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[[Category: Rna double helix]]
[[Category: Rna double helix]]
[[Category: Rna tandem g-a base pair]]
[[Category: Rna tandem g-a base pair]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:28:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:48:42 2008''

Revision as of 10:48, 27 July 2008

Image:1sa9.png

Template:STRUCTURE 1sa9

Crystal Structure of the RNA octamer GGCGAGCC

Template:ABSTRACT PUBMED 15103128

About this Structure

Full crystallographic information is available from OCA.

Reference

Structures of two RNA octamers containing tandem G.A base pairs., Jang SB, Baeyens K, Jeong MS, SantaLucia J Jr, Turner D, Holbrook SR, Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):829-35. Epub 2004, Apr 21. PMID:15103128

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