2hp4

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{{STRUCTURE_2hp4| PDB=2hp4 | SCENE= }}
{{STRUCTURE_2hp4| PDB=2hp4 | SCENE= }}
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'''Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor'''
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===Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor===
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==Overview==
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Human CD8 is a T cell coreceptor, which binds to pHLA I and plays a pivotal role in the activation of cytotoxic T lymphocytes. Soluble recombinant CD8 alphaalpha has been shown to antagonize T cell activation, both in vitro and in vivo. However, because of a very low affinity for pHLA I, high concentrations of soluble CD8 alphaalpha are required for efficient inhibition. Based upon our knowledge of the wild-type CD8/pHLA I structure, we have designed and produced a mutated form of soluble CD8 alphaalpha that binds to pHLA I with approximately fourfold higher affinity. We have characterized the binding of the high affinity CD8 mutant using surface plasmon resonance and determined its structure at 2.1 A resolution using X-ray crystallography. The analysis of this structure suggests that the higher affinity is achieved by providing a larger side chain that allows for an optimal contact to be made between the HLA alpha3 loop and the mutated CDR-like loops of CD8.
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(as it appears on PubMed at http://www.pubmed.gov), where 17243170 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17243170}}
==About this Structure==
==About this Structure==
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[[Category: Protein engineering]]
[[Category: Protein engineering]]
[[Category: Soluble protein]]
[[Category: Soluble protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:32:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:51:04 2008''

Revision as of 10:51, 27 July 2008

Image:2hp4.png

Template:STRUCTURE 2hp4

Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor

Template:ABSTRACT PUBMED 17243170

About this Structure

2HP4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Computational design and crystal structure of an enhanced affinity mutant human CD8 alphaalpha coreceptor., Cole DK, Rizkallah PJ, Boulter JM, Sami M, Vuidepot AL, Glick M, Gao F, Bell JI, Jakobsen BK, Gao GF, Proteins. 2007 Apr 1;67(1):65-74. PMID:17243170

Page seeded by OCA on Sun Jul 27 13:51:04 2008

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