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1snl

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[[Image:1snl.gif|left|200px]]
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{{STRUCTURE_1snl| PDB=1snl | SCENE= }}
{{STRUCTURE_1snl| PDB=1snl | SCENE= }}
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'''NMR Solution Structure of the Calcium-binding Domain of Nucleobindin (CALNUC)'''
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===NMR Solution Structure of the Calcium-binding Domain of Nucleobindin (CALNUC)===
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==Overview==
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Nucleobindin, also known as calnuc, participates in Ca2+ storage in the Golgi, as well as in other biological processes that involve DNA-binding and protein-protein interactions. We have determined the three-dimensional solution structure of the Ca(2+)-binding domain of nucleobindin by NMR showing that it consists of two EF-hand motifs. The NMR structure indicates that the phi and psi angles of residues in both motifs are very similar, despite the noncanonical sequence of the C-terminal EF-hand, which contains an arginine residue instead of the typical glycine at the sixth position of the 12-residue loop. The relative orientation of the alpha-helices in the N-terminal EF-hand falls within the common arrangement found in most EF-hand structures. In contrast, the noncanonical EF-hand deviates from the average orientation. The two helix-loop-helix moieties are in the open conformation characteristic of the Ca(2+)-bound state. We find that both motifs bind Ca2+ with apparent dissociation constants of 47 and 40 microM for the noncanonical and the canonical EF-hand, respectively. The Ca(2+)-binding domain of nucleobindin is unstructured in the absence of Ca2+ and folds upon Ca2+ addition. NMR relaxation data and structural studies of the folded domain indicate that it undergoes slow dynamics, suggesting that it is floppier and less compact than a globular domain.
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{{ABSTRACT_PUBMED_15287731}}
==About this Structure==
==About this Structure==
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1SNL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNL OCA].
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1SNL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNL OCA].
==Reference==
==Reference==
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[[Category: Calcium-binding]]
[[Category: Calcium-binding]]
[[Category: Ef-hand]]
[[Category: Ef-hand]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:55:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:59:30 2008''

Revision as of 10:59, 27 July 2008

Image:1snl.png

Template:STRUCTURE 1snl

NMR Solution Structure of the Calcium-binding Domain of Nucleobindin (CALNUC)

Template:ABSTRACT PUBMED 15287731

About this Structure

1SNL is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Structural studies on the Ca2+-binding domain of human nucleobindin (calnuc)., de Alba E, Tjandra N, Biochemistry. 2004 Aug 10;43(31):10039-49. PMID:15287731

Page seeded by OCA on Sun Jul 27 13:59:30 2008

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