2ltn

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{{STRUCTURE_2ltn| PDB=2ltn | SCENE= }}
{{STRUCTURE_2ltn| PDB=2ltn | SCENE= }}
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'''DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)'''
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===DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)===
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==Overview==
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The propeptide form of the lectin from the garden pea (Pisum sativum agglutinin) has been expressed in Escherichia coli by attaching its cDNA to an inducible promoter. By a number of criteria, including the ability to form dimers, hemagglutination titer, Western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. Preliminary crystallization experiments using the recombinant propeptide lectin yield crystals in space group P2(1)2(1)2(1) with a = 64.8 A, b = 73.8 A, and c = 109.0 A (1 A = 0.1 nm) that diffract to 2.8-A resolution. This unit cell size is quite similar to the unit cell determined for native pea lectin, suggesting that the overall structure of the recombinant prolectin is virtually identical.
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(as it appears on PubMed at http://www.pubmed.gov), where 2708344 is the PubMed ID number.
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{{ABSTRACT_PUBMED_2708344}}
==About this Structure==
==About this Structure==
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[[Category: Suddath, F L.]]
[[Category: Suddath, F L.]]
[[Category: Lectin]]
[[Category: Lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:10:10 2008''

Revision as of 11:10, 27 July 2008

Image:2ltn.png

Template:STRUCTURE 2ltn

DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)

Template:ABSTRACT PUBMED 2708344

About this Structure

2LTN is a Protein complex structure of sequences from Pisum sativum. Full crystallographic information is available from OCA.

Reference

Design, expression, and crystallization of recombinant lectin from the garden pea (Pisum sativum)., Prasthofer T, Phillips SR, Suddath FL, Engler JA, J Biol Chem. 1989 Apr 25;264(12):6793-6. PMID:2708344

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