1jyi

From Proteopedia

---

Revision as of 16:34, 20 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1jyi, resolution 2.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CONCANAVALIN A/12-MER PEPTIDE COMPLEX

Overview

The functional consequences of peptide-carbohydrate mimicry were analyzed, on the basis of the crystal structure of concanavalin A (ConA) in complex, with a carbohydrate-mimicking peptide, DVFYPYPYASGS. The peptide binds to, the non-crystallographically related monomers of two independent dimers of, ConA in two different modes, in slightly different conformations, demonstrating structural adaptability in ConA-peptide recognition. In one, mode, the peptide has maximum interactions with ConA, and in the other, it, shows relatively fewer contacts within this site but significant contacts, with the symmetry-related subunit. Neither of the peptide binding sites, overlaps with the structurally characterized mannose and trimannose, binding sites on ConA. Despite this, the functional mimicry between the, peptide and carbohydrate ligands was evident. The peptide-inhibited ConA, induced T cell proliferation in a dose-dependent manner. The effect of the, designed analogs of the peptide on ConA-induced T cell proliferation and, their recognition by the antibody response against alpha-d-mannopyranoside, indicate a role for aromatic residues in functional mimicry. Although the, functional mimicry was observed between the peptide and carbohydrate, moieties, the crystal structure of the ConA-peptide complex revealed that, the two peptide binding sites are independent of the methyl, alpha-d-mannopyranoside binding site.

About this Structure

1JYI is a Single protein structure of sequence from Canavalia ensiformis with MN and CA as ligands. Full crystallographic information is available from OCA.

Reference

Structural and functional consequences of peptide-carbohydrate mimicry. Crystal structure of a carbohydrate-mimicking peptide bound to concanavalin A., Jain D, Kaur K, Sundaravadivel B, Salunke DM, J Biol Chem. 2000 May 26;275(21):16098-102. PMID:10821862

Page seeded by OCA on Tue Nov 20 18:41:33 2007