2hkc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2hkc.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2hkc.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2hkc| PDB=2hkc | SCENE= }}
{{STRUCTURE_2hkc| PDB=2hkc | SCENE= }}
-
'''NMR Structure of the IQ-modified Dodecamer CTCGGC[IQ]GCCATC'''
+
===NMR Structure of the IQ-modified Dodecamer CTCGGC[IQ]GCCATC===
-
==Overview==
+
<!--
-
The solution structure of the oligodeoxynucleotide 5'-d(CTCGGCXCCATC)-3'.5'-d(GATGGCGCCGAG)-3' containing the heterocyclic amine 8-[(3-methyl-3H-imidazo[4,5-f]quinolin-2-yl)amino]-2'-deoxyguanosine adduct (IQ) at the third guanine in the NarI restriction sequence, a hot spot for -2 bp frameshifts, is reported. Molecular dynamics calculations restrained by distances derived from 24 (1)H NOEs between IQ and DNA, and torsion angles derived from (3)J couplings, yielded ensembles of structures in which the adducted guanine was displaced into the major groove with its glycosyl torsion angle in the syn conformation. One proton of its exocyclic amine was approximately 2.8 A from an oxygen of the 5' phosphodiester linkage, suggesting formation of a hydrogen bond. The carcinogen-guanine linkage was defined by torsion angles alpha' [N9-C8-N(IQ)-C2(IQ)] of 159 +/- 7 degrees and beta' [C8-N(IQ)-C2(IQ)-N3(IQ)] of -23 +/- 8 degrees . The complementary cytosine was also displaced into the major groove. This allowed IQ to intercalate between the flanking C.G base pairs. The disruption of Watson-Crick hydrogen bonding was corroborated by chemical-shift perturbations for base aromatic protons in the complementary strand opposite to the modified guanine. Chemical-shift perturbations were also observed for (31)P resonances corresponding to phosphodiester linkages flanking the adduct. The results confirmed that IQ adopted a base-displaced intercalated conformation in this sequence context but did not corroborate the formation of a hydrogen bond between the IQ quinoline nitrogen and the complementary dC [Elmquist, C. E.; Stover, J. S.; Wang, Z.; Rizzo, C. J. J. Am. Chem. Soc. 2004, 126, 11189-11201].
+
The line below this paragraph, {{ABSTRACT_PUBMED_16881637}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16881637 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16881637}}
==About this Structure==
==About this Structure==
-
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HKC OCA].
+
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HKC OCA].
==Reference==
==Reference==
Line 37: Line 41:
[[Category: Refinement]]
[[Category: Refinement]]
[[Category: Rmd calculation]]
[[Category: Rmd calculation]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:23:34 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:20:02 2008''

Revision as of 11:20, 27 July 2008

Image:2hkc.png

Template:STRUCTURE 2hkc

NMR Structure of the IQ-modified Dodecamer CTCGGC[IQ]GCCATC

Template:ABSTRACT PUBMED 16881637

About this Structure

Full experimental information is available from OCA.

Reference

Base-displaced intercalated structure of the food mutagen 2-amino-3-methylimidazo[4,5-f]quinoline in the recognition sequence of the NarI restriction enzyme, a hotspot for -2 bp deletions., Wang F, DeMuro NE, Elmquist CE, Stover JS, Rizzo CJ, Stone MP, J Am Chem Soc. 2006 Aug 9;128(31):10085-95. PMID:16881637

Page seeded by OCA on Sun Jul 27 14:20:02 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hkc"
Personal tools