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| | {{STRUCTURE_1wj9| PDB=1wj9 | SCENE= }} | | {{STRUCTURE_1wj9| PDB=1wj9 | SCENE= }} |
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| - | '''Crystal structure of a CRISPR-associated protein from thermus thermophilus'''
| + | ===Crystal structure of a CRISPR-associated protein from thermus thermophilus=== |
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| - | ==Overview==
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| - | We have determined the crystal structure of hypothetical protein TTHB192 from Thermus thermophilus HB8 at 1.9 A resolution. This protein is a member of the Escherichia coli ygcH sequence family, which contains approximately 15 sequence homologs of bacterial origin. These homologs have a high isoelectric point. The crystal structure reveals that TTHB192 consists of two independently folded domains, and that each domain exhibits a ferredoxin-like fold with a four-stranded antiparallel beta-sheet packed on one side by alpha-helices. These two tandem domains face each other to generate a beta-sheet platform. TTHB192 displays overall structural similarity to Sex-lethal protein and poly(A)-binding protein fragments. These proteins have RNA binding activity which is supported by a beta-sheet platform formed by two tandem repeats of an RNA recognition motif domain with signature sequence motifs on the beta-sheet surface. Although TTHB192 does not have the same signature sequence motif as the RNA recognition motif domain, the presence of an evolutionarily conserved basic patch on the beta-sheet platform could be functionally relevant for nucleic acid-binding. This report shows that TTHB192 and its sequence homologs adopt an RNA recognition motif-like domain and provides the first testable functional hypothesis for this protein family.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16672237}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16672237 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16672237}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Rsgi]] | | [[Category: Rsgi]] |
| | [[Category: Structural genomic]] | | [[Category: Structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:45:23 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:21:03 2008'' |
Revision as of 11:21, 27 July 2008
Template:STRUCTURE 1wj9
Crystal structure of a CRISPR-associated protein from thermus thermophilus
Template:ABSTRACT PUBMED 16672237
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structure of hypothetical protein TTHB192 from Thermus thermophilus HB8 reveals a new protein family with an RNA recognition motif-like domain., Ebihara A, Yao M, Masui R, Tanaka I, Yokoyama S, Kuramitsu S, Protein Sci. 2006 Jun;15(6):1494-9. Epub 2006 May 2. PMID:16672237
Page seeded by OCA on Sun Jul 27 14:21:03 2008
