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2axe

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{{STRUCTURE_2axe| PDB=2axe | SCENE= }}
{{STRUCTURE_2axe| PDB=2axe | SCENE= }}
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'''IODINATED COMPLEX OF ACETYL XYLAN ESTERASE AT 1.80 ANGSTROMS'''
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===IODINATED COMPLEX OF ACETYL XYLAN ESTERASE AT 1.80 ANGSTROMS===
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==Overview==
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Enzymatic and non-enzymatic iodination of the amino acid tyrosine is a well known phenomenon. The iodination technique has been widely used for labeling proteins. Using high-resolution X-ray crystallographic techniques, the chemical and three-dimensional structures of iodotyrosines formed by non-enzymatic incorporation of I atoms into tyrosine residues of a crystalline protein are described. Acetylxylan esterase (AXE II; 207 amino-acid residues) from Penicillium purpurogenum has substrate specificities towards acetate esters of D-xylopyranose residues in xylan and belongs to a new class of alpha/beta hydrolases. The crystals of the enzyme are highly ordered, tightly packed and diffract to better than sub-angstrom resolution at 85 K. The iodination technique has been utilized to prepare an isomorphous derivative of the AXE II crystal. The structure of the enzyme determined at 1.10 A resolution exclusively by normal and anomalous scattering from I atoms, along with the structure of the iodinated complex at 1.80 A resolution, demonstrate the formation of covalent bonds between I atoms and C atoms at ortho positions to the hydroxyl groups of two tyrosyl moieties, yielding iodotyrosines.
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(as it appears on PubMed at http://www.pubmed.gov), where 10089308 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10089308}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Iodotyrosine]]
[[Category: Iodotyrosine]]
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Revision as of 11:30, 27 July 2008

Image:2axe.png

Template:STRUCTURE 2axe

IODINATED COMPLEX OF ACETYL XYLAN ESTERASE AT 1.80 ANGSTROMS

Template:ABSTRACT PUBMED 10089308

About this Structure

2AXE is a Single protein structure of sequence from Penicillium purpurogenum. Full crystallographic information is available from OCA.

Reference

Determination of a protein structure by iodination: the structure of iodinated acetylxylan esterase., Ghosh D, Erman M, Sawicki M, Lala P, Weeks DR, Li N, Pangborn W, Thiel DJ, Jornvall H, Gutierrez R, Eyzaguirre J, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):779-84. PMID:10089308

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