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1s20

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{{STRUCTURE_1s20| PDB=1s20 | SCENE= }}
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'''A novel NAD binding protein revealed by the crystal structure of E. Coli 2,3-diketogulonate reductase (YiaK)'''
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===A novel NAD binding protein revealed by the crystal structure of E. Coli 2,3-diketogulonate reductase (YiaK)===
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==Overview==
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Escherichia coli YiaK catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH. It belongs to a large family of oxidoreductases that is conserved in archaea, bacteria, and eukaryotes but shows no sequence homology to other proteins. We report here the crystal structures at up to 2.0-A resolution of YiaK alone and in complex with NAD-tartrate. YiaK has a new polypeptide backbone fold and a novel mode of recognizing the NAD cofactor. In addition, NAD is bound in an unusual conformation, at the interface of a dimer of the enzyme. The crystallographic analysis unexpectedly revealed the binding of tartrate in the active site. Enzyme kinetics studies confirm that tartrate and the related D-malate are inhibitors of YiaK. In contrast to most other enzymes where substrate binding produces a more closed conformation, the binding of NAD-tartrate to YiaK produces a more open active site. The free enzyme conformation is incompatible with NAD binding. His(44) is likely the catalytic residue of the enzyme.
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{{ABSTRACT_PUBMED_14718529}}
==About this Structure==
==About this Structure==
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[[Category: Xiao, R.]]
[[Category: Xiao, R.]]
[[Category: Alpha beta dimeric protein]]
[[Category: Alpha beta dimeric protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:11:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:35:00 2008''

Revision as of 11:35, 27 July 2008

Image:1s20.png

Template:STRUCTURE 1s20

A novel NAD binding protein revealed by the crystal structure of E. Coli 2,3-diketogulonate reductase (YiaK)

Template:ABSTRACT PUBMED 14718529

About this Structure

1S20 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A novel NAD-binding protein revealed by the crystal structure of 2,3-diketo-L-gulonate reductase (YiaK)., Forouhar F, Lee I, Benach J, Kulkarni K, Xiao R, Acton TB, Montelione GT, Tong L, J Biol Chem. 2004 Mar 26;279(13):13148-55. Epub 2004 Jan 12. PMID:14718529

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