1pz2

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{{STRUCTURE_1pz2| PDB=1pz2 | SCENE= }}
{{STRUCTURE_1pz2| PDB=1pz2 | SCENE= }}
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'''Crystal structure of a transient covalent reaction intermediate of a family 51 alpha-L-arabinofuranosidase'''
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===Crystal structure of a transient covalent reaction intermediate of a family 51 alpha-L-arabinofuranosidase===
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==Overview==
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High-resolution crystal structures of alpha-L-arabinofuranosidase from Geobacillus stearothermophilus T-6, a family 51 glycosidase, are described. The enzyme is a hexamer, and each monomer is organized into two domains: a (beta/alpha)8-barrel and a 12-stranded beta sandwich with jelly-roll topology. The structures of the Michaelis complexes with natural and synthetic substrates, and of the transient covalent arabinofuranosyl-enzyme intermediate represent two stable states in the double displacement mechanism, and allow thorough examination of the catalytic mechanism. The arabinofuranose sugar is tightly bound and distorted by an extensive network of hydrogen bonds. The two catalytic residues are 4.7 A apart, and together with other conserved residues contribute to the stabilization of the oxocarbenium ion-like transition state via charge delocalization and specific protein-substrate interactions. The enzyme is an anti-protonator, and a 1.7 A electrophilic migration of the anomeric carbon takes place during the hydrolysis.
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The line below this paragraph, {{ABSTRACT_PUBMED_14517232}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 14517232 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14517232}}
==About this Structure==
==About this Structure==
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[[Category: Shoham, Y.]]
[[Category: Shoham, Y.]]
[[Category: Beta-alpha8-barrel]]
[[Category: Beta-alpha8-barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:40:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:48:45 2008''

Revision as of 11:48, 27 July 2008

Image:1pz2.png

Template:STRUCTURE 1pz2

Crystal structure of a transient covalent reaction intermediate of a family 51 alpha-L-arabinofuranosidase

Template:ABSTRACT PUBMED 14517232

About this Structure

1PZ2 is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

Reference

Crystal structure and snapshots along the reaction pathway of a family 51 alpha-L-arabinofuranosidase., Hovel K, Shallom D, Niefind K, Belakhov V, Shoham G, Baasov T, Shoham Y, Schomburg D, EMBO J. 2003 Oct 1;22(19):4922-32. PMID:14517232

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