1qfz

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[[Image:1qfz.gif|left|200px]]
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{{Seed}}
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[[Image:1qfz.png|left|200px]]
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{{STRUCTURE_1qfz| PDB=1qfz | SCENE= }}
{{STRUCTURE_1qfz| PDB=1qfz | SCENE= }}
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'''PEA FNR Y308S MUTANT IN COMPLEX WITH NADPH'''
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===PEA FNR Y308S MUTANT IN COMPLEX WITH NADPH===
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==Overview==
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The flavoenzyme ferredoxin-NADP+ reductase (FNR) catalyzes the production of NADPH during photosynthesis. Whereas the structures of FNRs from spinach leaf and a cyanobacterium as well as many of their homologs have been solved, none of these studies has yielded a productive geometry of the flavin-nicotinamide interaction. Here, we show that this failure occurs because nicotinamide binding to wild type FNR involves the energetically unfavorable displacement of the C-terminal Tyr side chain. We used mutants of this residue (Tyr 308) of pea FNR to obtain the structures of productive NADP+ and NADPH complexes. These structures reveal a unique NADP+ binding mode in which the nicotinamide ring is not parallel to the flavin isoalloxazine ring, but lies against it at an angle of approximately 30 degrees, with the C4 atom 3 A from the flavin N5 atom.
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(as it appears on PubMed at http://www.pubmed.gov), where 10467097 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10467097}}
==About this Structure==
==About this Structure==
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[[Category: Hydride transfer]]
[[Category: Hydride transfer]]
[[Category: Photosynthesis]]
[[Category: Photosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:13:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:49:38 2008''

Revision as of 11:49, 27 July 2008

Image:1qfz.png

Template:STRUCTURE 1qfz

PEA FNR Y308S MUTANT IN COMPLEX WITH NADPH

Template:ABSTRACT PUBMED 10467097

About this Structure

1QFZ is a Single protein structure of sequence from Pisum sativum. Full crystallographic information is available from OCA.

Reference

A productive NADP+ binding mode of ferredoxin-NADP + reductase revealed by protein engineering and crystallographic studies., Deng Z, Aliverti A, Zanetti G, Arakaki AK, Ottado J, Orellano EG, Calcaterra NB, Ceccarelli EA, Carrillo N, Karplus PA, Nat Struct Biol. 1999 Sep;6(9):847-53. PMID:10467097

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