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2ber

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[[Image:2ber.gif|left|200px]]
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{{STRUCTURE_2ber| PDB=2ber | SCENE= }}
{{STRUCTURE_2ber| PDB=2ber | SCENE= }}
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'''Y370G ACTIVE SITE MUTANT OF THE SIALIDASE FROM MICROMONOSPORA VIRIDIFACIENS IN COMPLEX WITH BETA-NEU5AC (SIALIC ACID).'''
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===Y370G ACTIVE SITE MUTANT OF THE SIALIDASE FROM MICROMONOSPORA VIRIDIFACIENS IN COMPLEX WITH BETA-NEU5AC (SIALIC ACID).===
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==Overview==
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Mutagenesis of the conserved tyrosine (Y370) of the Micromonospora viridifaciens sialidase to small amino acids changes the mechanism of catalysis from retention of anomeric configuration to inversion [Watson, J. N., et al. (2003) Biochemistry 42, 12682-12690]. For the Y370G mutant enzyme-catalyzed hydrolysis of a series of aryl sialosides and 3'-sialyllactose, the derived Bronsted parameters (beta(lg)) on k(cat) and k(cat)/K(m) are -0.63 +/- 0.05 and -0.80 +/- 0.08, respectively. Thus, for the Y370G enzyme, glycosidic C-O bond cleavage is rate-determining. Analysis of the activity of the Y370G mutant and wild-type enzymes against a substrate [3,4-dihydro-2H-pyrano[3,2-c]pyridinium alpha-d-N-acetylneuraminide (DHP-alphaNeu5Ac)] whose hydrolysis cannot be accelerated by acid catalysis is consistent with these reactions proceeding via S(N)1 and S(N)2 mechanisms, respectively. The overall structure of the Y370G mutant sialidase active site is very similar to the previously reported wild-type structure [Gaskell, A., et al. (1995) Structure 3, 1197-1205], although removal of the tyrosine residue creates two significant changes to the active site. First, the anomeric oxygen atom of the hydrolysis product (beta-N-acetylneuraminic acid) and four water molecules bind in the large cavity created by the Y370G mutation. Second, the side chain of Asn310 moves to make a strong hydrogen bond to one of the bound water molecules.
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{{ABSTRACT_PUBMED_15966735}}
==About this Structure==
==About this Structure==
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[[Category: Micromonospora viridifacien]]
[[Category: Micromonospora viridifacien]]
[[Category: Sialidase]]
[[Category: Sialidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:11:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:53:48 2008''

Revision as of 11:53, 27 July 2008

Image:2ber.png

Template:STRUCTURE 2ber

Y370G ACTIVE SITE MUTANT OF THE SIALIDASE FROM MICROMONOSPORA VIRIDIFACIENS IN COMPLEX WITH BETA-NEU5AC (SIALIC ACID).

Template:ABSTRACT PUBMED 15966735

About this Structure

2BER is a Single protein structure of sequence from Micromonospora viridifaciens. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of action of an inverting mutant sialidase., Newstead S, Watson JN, Knoll TL, Bennet AJ, Taylor G, Biochemistry. 2005 Jun 28;44(25):9117-22. PMID:15966735

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