2ezv

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[[Image:2ezv.gif|left|200px]]
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[[Image:2ezv.png|left|200px]]
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{{STRUCTURE_2ezv| PDB=2ezv | SCENE= }}
{{STRUCTURE_2ezv| PDB=2ezv | SCENE= }}
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'''Crystal structure of tetrameric restriction endonuclease SfiI bound to cognate DNA.'''
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===Crystal structure of tetrameric restriction endonuclease SfiI bound to cognate DNA.===
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==Overview==
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Many reactions in cells proceed via the sequestration of two DNA molecules in a synaptic complex. SfiI is a member of a growing family of restriction enzymes that can bind and cleave two DNA sites simultaneously. We present here the structures of tetrameric SfiI in complex with cognate DNA. The structures reveal two different binding states of SfiI: one with both DNA-binding sites fully occupied and the other with fully and partially occupied sites. These two states provide details on how SfiI recognizes and cleaves its target DNA sites, and gives insight into sequential binding events. The SfiI recognition sequence (GGCCNNNN[downward arrow]NGGCC) is a subset of the recognition sequence of BglI (GCCNNNN[downward arrow]NGGC), and both enzymes cleave their target DNAs to leave 3-base 3' overhangs. We show that even though SfiI is a tetramer and BglI is a dimer, and there is little sequence similarity between the two enzymes, their modes of DNA recognition are unusually similar.
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The line below this paragraph, {{ABSTRACT_PUBMED_16308566}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16308566 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16308566}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA., Vanamee ES, Viadiu H, Kucera R, Dorner L, Picone S, Schildkraut I, Aggarwal AK, EMBO J. 2005 Dec 7;24(23):4198-208. Epub 2005 Nov 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16308566 16308566]
A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA., Vanamee ES, Viadiu H, Kucera R, Dorner L, Picone S, Schildkraut I, Aggarwal AK, EMBO J. 2005 Dec 7;24(23):4198-208. Epub 2005 Nov 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16308566 16308566]
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Crystallization of restriction endonuclease SfiI in complex with DNA., Viadiu H, Vanamee ES, Jacobson EM, Schildkraut I, Aggarwal AK, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1493-5. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876363 12876363]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Type II site-specific deoxyribonuclease]]
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[[Category: Protein/dna complex]]
[[Category: Protein/dna complex]]
[[Category: Type iif restriction endonuclease]]
[[Category: Type iif restriction endonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:17:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:58:15 2008''

Revision as of 11:58, 27 July 2008

Image:2ezv.png

Template:STRUCTURE 2ezv

Crystal structure of tetrameric restriction endonuclease SfiI bound to cognate DNA.

Template:ABSTRACT PUBMED 16308566

About this Structure

2EZV is a Single protein structure. Full crystallographic information is available from OCA.

Reference

A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA., Vanamee ES, Viadiu H, Kucera R, Dorner L, Picone S, Schildkraut I, Aggarwal AK, EMBO J. 2005 Dec 7;24(23):4198-208. Epub 2005 Nov 24. PMID:16308566

Crystallization of restriction endonuclease SfiI in complex with DNA., Viadiu H, Vanamee ES, Jacobson EM, Schildkraut I, Aggarwal AK, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1493-5. Epub 2003, Jul 23. PMID:12876363

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