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| - | [[Image:1y19.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1y19| PDB=1y19 | SCENE= }} | | {{STRUCTURE_1y19| PDB=1y19 | SCENE= }} |
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| - | '''Structural basis for phosphatidylinositol phosphate kinase type I-gamma binding to talin at focal adhesions'''
| + | ===Structural basis for phosphatidylinositol phosphate kinase type I-gamma binding to talin at focal adhesions=== |
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| - | ==Overview==
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| - | The cytoskeletal protein talin binds to a short C-terminal sequence in phosphatidylinositol phosphate kinase type Igamma (PIPKIgamma), activating the enzyme and promoting the local production of phosphatidylinositol 4,5 bisphosphate, which regulates focal adhesion dynamics as well as clathrin-mediated endocytosis in neuronal cells. Here we show by crystallographic, NMR, and calorimetric analysis that the phosphotyrosine binding (PTB)-like domain of talin engages the PIPKIgamma C terminus in a mode very similar to that of integrin binding. However, PIPKIgamma binds in the canonical PTB-peptide mode with an SPLH motif replacing the classic NPXY motif. The tighter packing of the SPLH motif against the hydrophobic core of talin may explain the stronger binding of PIPKIgamma. Two tyrosine residues flanking the SPLH motif (Tyr-644 and Tyr-649) have been implicated in the regulation of talin binding. We show that phosphorylation at Tyr-644, a Src phosphorylation site in vivo, has little effect on the binding mode or strength, which is consistent with modeling studies in which the phosphotyrosine makes surface-exposed salt bridges, and we suggest that its strong activating effect arises from the release of autoinhibitory restraints in the full-length PIPKIgamma. Modeling studies suggest that phosphorylation of Tyr-649 will likewise have little effect on talin binding, whereas phosphorylation of the SPLH serine is predicted to be strongly disruptive. Our data are consistent with the proposal that Src activity promotes a switch from integrin binding to PIPKIgamma binding that regulates focal adhesion turnover. | + | The line below this paragraph, {{ABSTRACT_PUBMED_15623515}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15623515 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15623515}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Npxy motif]] | | [[Category: Npxy motif]] |
| | [[Category: Ptb domain]] | | [[Category: Ptb domain]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:45:44 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:04:51 2008'' |
Revision as of 12:04, 27 July 2008
Template:STRUCTURE 1y19
Structural basis for phosphatidylinositol phosphate kinase type I-gamma binding to talin at focal adhesions
Template:ABSTRACT PUBMED 15623515
About this Structure
1Y19 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis for phosphatidylinositol phosphate kinase type Igamma binding to talin at focal adhesions., de Pereda JM, Wegener KL, Santelli E, Bate N, Ginsberg MH, Critchley DR, Campbell ID, Liddington RC, J Biol Chem. 2005 Mar 4;280(9):8381-6. Epub 2004 Dec 28. PMID:15623515
Page seeded by OCA on Sun Jul 27 15:04:51 2008
